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Journal article
Roles of a Y-Linked iDmrt1 Paralogue and Insulin-like Androgenic Gland Hormone in Sexual Development in the Tropical Rock Lobster, Panulirus ornatus
International Journal of Molecular Sciences, Vol.26(11), pp.1-20
2025
PMID: 40507961
Abstract
Understanding the mechanisms of sexual development would pave the way for producing mono-sex populations to aid the aquaculture industry. This study investigates the functions of the Y-linked iDmrt1 paralogue (Po-iDMY) and insulin-like androgenic gland hormone (Po-IAG) in the process of sexual development in the tropical rock lobster, Panulirus ornatus (TRL). Previously, we identified that Po-iDMY, a male-specific heterogametic (Y-linked) paralogue of the autosomal Po-iDmrt1 found in TRL, is a second sex-linked iDmrt gene identified in invertebrates. Using 5′ and 3′ rapid amplification of cDNA ends and data from a draft male genome (with an assembly genome size of approximately 2.446 Gbp and 87% BUSCO completeness), we obtained the full-length Po-iDMY gene (encoding a protein of 312 amino acids). A 411 bp male-specific sequence located at the 3′ untranslated region of Po-iDMY mRNA was used as a sex marker, which was reported for the first time in our draft genome. However, Po-iDMY is not a master sex-determining factor since it was not expressed across developmental stages of embryos, juveniles and adults. Instead, we silenced Po-IAG at an early juvenile stage, generating two potential neo-females, implying that sexual manipulation could be a promising technique in TRL.
Details
- Title
- Roles of a Y-Linked iDmrt1 Paralogue and Insulin-like Androgenic Gland Hormone in Sexual Development in the Tropical Rock Lobster, Panulirus ornatus
- Authors
- Ai Hang Tran Nguyen - University of the Sunshine Coast, Queensland, Centre for BioinnovationJihye Yoon - University of TasmaniaWenyan Nong - Chinese University of Hong KongSusan Glendinning - University of the Sunshine Coast, Queensland, Centre for BioinnovationQuinn P Fitzgibbon - University of TasmaniaGregory G Smith - University of TasmaniaJerome H L Hui - Chinese University of Hong KongKa Hou Chu - Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)Volker Herzig - University of the Sunshine Coast, Queensland, Centre for BioinnovationTomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- International Journal of Molecular Sciences, Vol.26(11), pp.1-20
- Publisher
- MDPI AG
- Date published
- 2025
- DOI
- 10.3390/ijms26115149
- ISSN
- 1422-0067; 1661-6596
- PMID
- 40507961
- Data Availability
- Data is contained within the article and Supplementary Materials.
- Organisation Unit
- School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991136299202621
- Output Type
- Journal article
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- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
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