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Roles of a Y-linked iDmrt1 paralogue and Insulin-Like Androgenic Gland Hormone in Sexual Development in the Tropical Rock Lobster, Panulirus ornatus
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Roles of a Y-linked iDmrt1 paralogue and Insulin-Like Androgenic Gland Hormone in Sexual Development in the Tropical Rock Lobster, Panulirus ornatus

Hang Tran Nguyen Ai, Jihye Yoon, Wenyan Nong, Susan Glendinning, Gregory Smith, Quinn P. Fitzgibbon, Jerome H.L. Hui, Ka-Hou Chu, Volker Herzig and Tomer Ventura
Figshare
Digital Science
2024
DOI: https://doi.org/10.6084/m9.figshare.28039316.v2
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https://doi.org/10.6084/m9.figshare.28039316View
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Abstract

master sex determinant Dmrt iDMY IAG transactivation domain Crustacea
This study investigates the functions of the Y-linked iDmrt1 paralogue, Po-iDMY and insulin-like androgenic gland hormone (Po-IAG) in the process of sexual development in the tropical rock lobster, Panulirus ornatus (TRL). Previously, we identified that Po-iDMY, a male-specific heterogametic (Y-linked) paralogue of the autosomal Po-iDmrt1 found in TRL, is a second sex-linked iDmrt gene identified in invertebrates. Using 5’ and 3’ rapid amplification of cDNA ends and data from a draft male genome (with an assembly genome size of approximately 2.446 Gbp and 87% BUSCO completeness), we obtained the full-length Po-iDMY gene (coding for a 312 amino acids protein), which includes a 411 bp male-specific sequence located at the 3’ untranslated region of Po-iDMY mRNA used as a sex marker. Notably, this sex marker sequence is reported for the first time in our genome analysis. However, Po-iDMY is not expressed across various tissues and developmental stages across embryos, juveniles and adults. This observation suggests that Po-iDMY may not tightly regulate Po-IAG in this species, indicating that silencing Po-IAG could potentially induce sex change. Further expression analyses confirmed that Po-IAG expression is not male-specific. Despite this, Po-IAG silencing at an early juvenile stage generated two potential neo-females, implying that sexual manipulation could be a promising technique in TRL.

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