Manila clam, Ruditapes philippinarum Cathepsin D: Molecular analysis and immune response against brown ring disease causing Vibrio tapetis challenge

Udeni Menike; Krishan Ariyasiri; Jin-Young Choi; Youngdeuk Lee; W. D. N. Wickramaarachchi; H.K.A. Premachandra; Jehee Lee; Mahanama De Zoysa

doi:10.9710/kjm.2013.29.2.155

Back

Manila clam, Ruditapes philippinarum Cathepsin D: Molecular analysis and immune response against brown ring disease causing Vibrio tapetis challenge

Journal article

Open access

Peer reviewed

Manila clam, Ruditapes philippinarum Cathepsin D: Molecular analysis and immune response against brown ring disease causing Vibrio tapetis challenge

Udeni Menike, Krishan Ariyasiri, Jin-Young Choi, Youngdeuk Lee, W. D. N. Wickramaarachchi, H.K.A. Premachandra, Jehee Lee and Mahanama De Zoysa

Korean Journal of Malacology [Han-gug Paeryu Hag-hoeji], Vol.29(2), pp.155-161

2013

DOI: https://doi.org/10.9710/kjm.2013.29.2.155

Files and links (1)

pdf

MCcathepsinD540.06 kBDownload View

Published VersionCC BY-NC V4.0, Open Access

Abstract

Immunogenetics (incl. genetic immunology)

Fish physiology and genetics

Manila clam (Ruditapes philipphinarum)

Brown ring disease

Cathepsin D

tapetis

Cathepsins are lysosomal/cysteine proteases belong to papain family (C1 family) that is involved in intracellular protein degradation, antigen processing, hormone maturation, and immune responses. In this study, member of cathepsin family was identified from Manila clam (Mc-Cathepsin D) and investigated the immune response against brown ring disease (BRD) causing Vibrio tapetis challenge. The identified Mc-Cathepsin D gene encodes characteristic features typical for the cathepsin family including eukaryotic and viral aspartyl protease signature domain and two highly conserved active sites ($^{84}VVFDTGSSNLWV^{95}$ and $^{270}IADTGTSLLAG^{281}$). Moreover, MC-Cathepsin D shows higher identity values (-50-70%) and conserved amino acids with known cathepsin D members. Transcriptional results (by quantitative real-time RT-PCR) showed that Mc-Cathepsin D was expressed at higher levels in gills and hemocytes than mantle, adductor muscle, foot, and siphon. After the V. tapetis challenge under laboratory conditions, Mc-Cathepsin D mRNA was up-regulated in gills and hemocytes. Present study indicates that Mc-Cathepsin D is constitutively expressed in different tissues and potentially inducible when infecting BRD by V. tapetis. It is further suggesting that Mc-Cathepsin D may be involved in multiple role including immune response reactions against BRD.

Details

Title: Manila clam, Ruditapes philippinarum Cathepsin D: Molecular analysis and immune response against brown ring disease causing Vibrio tapetis challenge
Authors: Udeni Menike - Chungnam National University
Krishan Ariyasiri - Chungnam National University
Jin-Young Choi - Chungnam National University
Youngdeuk Lee - Korea Institute of Ocean Science and Technology
W. D. N. Wickramaarachchi - Jeju National University
H.K.A. Premachandra - Jeju National University
Jehee Lee - Jeju National University
Mahanama De Zoysa (Corresponding Author) - Chungnam National University
Publication details: Korean Journal of Malacology [Han-gug Paeryu Hag-hoeji], Vol.29(2), pp.155-161
Publisher: Han-gug Paeryu Hag-hoe | Malacological Society of Korea
Date published: 2013
DOI: 10.9710/kjm.2013.29.2.155
ISSN: 1225-3480
Copyright note: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License with permits unrestricted non-commercial use, distribution, and reproducibility in any medium, provided the original work is properly cited.
Grant note: This study was financially supported by research fund of Chungnam National University in 2011.
Organisation Unit: Centre for Bioinnovation
Language: English
Record Identifier: 991206780402621
Output Type: Journal article

Metrics

1 Record Views