Preprint
A cell cycle-coordinated nuclear compartment for Polymerase II transcription encompasses the earliest gene expression before global genome activation
bioRxiv
Cold Spring Harbor Laboratory Press
2018
Abstract
Most metazoan embryos commence development with rapid cleavages without zygotic gene expression and their genome activation is delayed until the mid-blastula transition (MBT). However, a set of genes escape global repression during the extremely fast cell cycles, which lack gap phases and their transcription is activated before the MBT. Here we describe the formation and the spatio-temporal dynamics of a distinct transcription compartment, which encompasses the earliest detectable transcription during the first wave of genome activation. Simultaneous 4D imaging of expression of pri-miR430 and zinc finger genes by a novel, native transcription imaging approach reveals a pair of shared transcription compartments regulated by homolog chromosome organisation. These nuclear compartments carry the majority of nascent RNAs and transcriptionally active Polymerase II, are depleted of compact chromatin and represent the main sites for detectable transcription before MBT. We demonstrate that transcription occurs in the S-phase of the cleavage cycles and that the gradual slowing of these cell cycles are permissive to transcription before global genome activation. We propose that the demonstrated transcription compartment is part of the regulatory architecture of nucleus organisation, and provides a transcriptionally competent, supporting environment to facilitate early escape from the general nuclear repression before global genome activation.
Details
- Title
- A cell cycle-coordinated nuclear compartment for Polymerase II transcription encompasses the earliest gene expression before global genome activation
- Authors
- Yavor Hadzhiev (Author) - University of BirminghamHaseeb K Qureshi (Author) - University of BirminghamLucy Wheatley (Author) - University of BirminghamLedean Cooper (Author) - University of BirminghamAleksandra Jasiulewicz (Author) - University of BirminghamHuy Van Nguyen (Author) - University of BirminghamJoseph Wragg (Author) - University of BirminghamDivyasree Poovathumkadavil (Author) - University of BirminghamSascha Conic (Author) - University of StrasbourgSarah Bajan (Author) - University of Technology SydneyAttila Sik (Author) - University of BirminghamGyörgy Hutvàgner (Author) - University of Technology SydneyLàszlò Tora (Author) - University of StrasbourgAgnieszka Gambus (Author) - University of BirminghamJohn S Fossey (Author) - University of BirminghamFerenc Müller (Author) - University of Birmingham
- Publication details
- bioRxiv
- Publisher
- Cold Spring Harbor Laboratory Press
- DOI
- 10.1101/366468
- ISSN
- 2692-8205
- Organisation Unit
- School of Health and Behavioural Sciences - Legacy; School of Health and Sport Sciences - Legacy; University of the Sunshine Coast, Queensland; School of Health - Biomedicine
- Language
- English
- Record Identifier
- 99472506402621
- Output Type
- Preprint
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