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A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation4.11 MB
Published VersionCC BY V4.0, Open Access
Journal article
A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation
Nature communications, Vol.10(1), pp.1-14
2019
PMID: 30741925
Abstract
Most metazoan embryos commence development with rapid, transcriptionally silent cell divisions, with genome activation delayed until the mid-blastula transition (MBT). However, a set of genes escapes global repression and gets activated before MBT. Here we describe the formation and the spatio-temporal dynamics of a pair of distinct transcription compartments, which encompasses the earliest gene expression in zebrafish. 4D imaging of pri-miR430 and zinc-finger-gene activities by a novel, native transcription imaging approach reveals transcriptional sharing of nuclear compartments, which are regulated by homologous chromosome organisation. These compartments carry the majority of nascent-RNAs and active Polymerase II, are chromatin-depleted and represent the main sites of detectable transcription before MBT. Transcription occurs during the S-phase of increasingly permissive cleavage cycles. It is proposed, that the transcription compartment is part of the regulatory architecture of embryonic nuclei and offers a transcriptionally competent environment to facilitate early escape from repression before global genome activation.
Details
- Title
- A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation
- Authors
- Yavor Hadzhiev (Author) - University of BirminghamHaseeb K Qureshi (Author) - University of BirminghamLucy Wheatley (Author) - University of BirminghamLedean Cooper (Author) - University of BirminghamAleksandra Jasiulewicz (Author) - University of BirminghamHuy Van Nguyen (Author) - University of BirminghamJoseph W Wragg (Author) - University of BirminghamDivyasree Poovathumkadavil (Author) - University of BirminghamSascha Conic (Author) - Université de StrasbourgSarah Bajan (Author) - University of Technology SydneyAttila Sik (Author) - University of BirminghamGyörgy Hutvàgner (Author) - University of Technology SydneyLàszlò Tora (Author) - Université de StrasbourgAgnieszka Gambus (Author) - University of BirminghamJohn S Fossey (Author) - University of BirminghamFerenc Müller (Author) - University of Birmingham
- Publication details
- Nature communications, Vol.10(1), pp.1-14
- Publisher
- Nature Publishing Group
- Date published
- 2019
- DOI
- 10.1038/s41467-019-08487-5
- ISSN
- 2041-1723
- PMID
- 30741925
- Copyright note
- Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99472506702621
- Output Type
- Journal article
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