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The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype4.99 MB
Preprint VersionCC BY-NC V4.0, Open Access
Preprint
The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
bioRxiv
Cold Spring Harbor Laboratory Press
2020
Appears in Cancer Research Cluster Research Collection
Abstract
It is now appreciated that long non-coding RNAs (lncRNAs) are important players in the orchestration of cancer progression. In this study we characterized GHSROS, a human lncRNA gene on the opposite DNA strand (antisense) to the ghrelin receptor gene, in prostate cancer. The lncRNA was upregulated by prostate tumors from different clinical datasets. Consistently, transcriptome data revealed that GHSROS alters the expression of cancer-associated genes. Functional analyses in vitro showed that GHSROS mediates tumor growth, migration, and survival and resistance to the cytotoxic drug docetaxel. Increased cellular proliferation of GHSROS-overexpressing PC3, DU145, and LNCaP prostate cancer cell lines in vitro was recapitulated in a subcutaneous xenograft model. Conversely, in vitro antisense oligonucleotide inhibition of the lncRNA reciprocally regulated cell growth and migration, and gene expression. Notably, GHSROS modulates the expression of PPP2R2C, the loss of which may drive androgen receptor pathway-independent prostate tumor progression in a subset of prostate cancers. Collectively, our findings suggest that GHSROS can reprogram prostate cancer cells toward a more aggressive phenotype and that this lncRNA may represent a potential therapeutic target.
Details
- Title
- The long non-coding RNA GHSROS reprograms prostate cancer cell lines toward a more aggressive phenotype
- Authors
- Patrick Thomas (Author) - Queensland University of TechnologyPenny L Jeffery (Author) - Queensland University of TechnologyM D Gahete (Author) - Universidad Nacional de CórdobaE Whiteside (Author) - University of Southern QueenslandCarina Walpole (Author) - Queensland University of TechnologyMichelle Maugham (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyLidija Jovanovic (Author) - Queensland University of TechnologyJennifer H Gunter (Author) - Queensland University of TechnologyElizabeth D Williams (Author) - Queensland University of TechnologyColleen C Nelson (Author) - Queensland University of TechnologyA C Herington (Author) - Queensland University of TechnologyR M Luque (Author) - University of CórdobaR N Veedu (Author) - Murdoch UniversityL Chopin (Author) - Queensland University of TechnologyI Seim (Author) - Queensland University of Technology
- Publication details
- bioRxiv
- Publisher
- Cold Spring Harbor Laboratory Press
- Date published
- 2020
- DOI
- 10.1101/682203
- ISSN
- 2692-8205
- Copyright note
- Copyright © 2020 The authors. This preprint (which was not peer-reviewed) is made available under a CC-BY-NC 4.0 International license.
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; Cancer Research Cluster; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99492808502621
- Output Type
- Preprint
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