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HPV16 E6-178G E7-647G Promotes Proliferation and Inhibits Apoptosis in Cervical Cancer C33A Cells447.81 kB
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Preprint
HPV16 E6-178G/E7-647G Promotes Proliferation and Inhibits Apoptosis in Cervical Cancer C33A Cells
Research Square, Vol.28 January 2021, pp.1-12
Research Square Company
2021
Abstract
Background: HPV16 is the main cause of cervical cancer. In our study, we aimed to investigate the role of HPV mutants HPV16 E6-178G/E7-647G in the proliferation and apoptosis of cervical cancer C33A cells. Methods: Plasmids encoding the HPV16 E7 prototype (E7-647A)-GV144, E7 mutant (E7-647G)-GV144, HPV16 E6/E7 prototype (E6-178T/E7-647A)-GV144, and E6/E7 mutant (E6-178G/E7-647G)-GV144 were stably transfected into cervical cancer C33A cells. Western blot analysis, CCK8 proliferation assay, cell cloning assay and ow cytometry were used to detect the effects of the different polymorphism sites in HPV16 on cell proliferation and apoptosis. Results: HPV16 mutations promoted the proliferation and inhibited the apoptosis of cervical cancer C33A cells, and the effect of the E6-178G/E7-647G co-mutation was signicantly greater than that of the single E7- 647G mutant (P<0.05). Conclusions: HPV16 E6-178G/E7-647G can thus promote the proliferation and inhibit the apoptosis of cervical cancer cells. Importance: HPV16 is the most common type and the main cause of cervical cancer. It is a fatal threat to the health of millions of women. The genetic differences among HPV16 sub-types may be related to their carcinogenic potential. HPV mutations can differ in biology and etiology, leading to differences in tumor development and behavior. However, little is known about the carcinogenic potential of the HPV16 variant in Asian women compared to many studies in European and American populations.
Details
- Title
- HPV16 E6-178G/E7-647G Promotes Proliferation and Inhibits Apoptosis in Cervical Cancer C33A Cells
- Authors
- Xiangyi Zhe (Author) - Shihezi UniversityChunhe Zhang (Author) - Shihezi UniversityHuizhen Xin (Author) - Shihezi UniversityZhenzhen Pan (Author) - Xinjiang Production and Construction CorpsDongmei Li (Author) - Shihezi UniversityHongtao Li (Author) - Shihezi UniversityFuyuan Jin (Author) - Shihezi UniversityHaichen Long (Author) - Shihezi UniversityWeinan Zheng (Author) - Chengdu Medical CollegeRenfu Shao (Author) - University of the Sunshine Coast, Queensland, School of Science and Engineering - LegacyZemin Pan (Author) - Shihezi University
- Publication details
- Research Square, Vol.28 January 2021, pp.1-12
- Publisher
- Research Square Company
- Date published
- 2021
- DOI
- 10.21203/rs.3.rs-153936/v1
- ISSN
- 2693-5015
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99509508302621
- Output Type
- Preprint
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