emm and C-Repeat Region Molecular Typing of Beta-Hemolytic Streptococci in a Tropical Country: Implications for Vaccine Development

A C Steer; G Magor; A W J Jenney; J Kado; M F Good; David J McMillan; M Batzloff; J R Carapetis

doi:10.1128/JCM.00312-09

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emm and C-Repeat Region Molecular Typing of Beta-Hemolytic Streptococci in a Tropical Country: Implications for Vaccine Development

Journal article

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emm and C-Repeat Region Molecular Typing of Beta-Hemolytic Streptococci in a Tropical Country: Implications for Vaccine Development

A C Steer, G Magor, A W J Jenney, J Kado, M F Good, David J McMillan, M Batzloff and J R Carapetis

Journal of Clinical Microbiology, Vol.47(8), pp.2502-2509

2009

DOI: https://doi.org/10.1128/JCM.00312-09

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Abstract

We designed a study to investigate the molecular epidemiology of group A streptococcal (GAS) and group C and G streptococcal (GCS and GGS) disease in Fiji, a country which is known to have a high burden of streptococcal disease. Molecular typing of the N-terminal portion (emm typing) of the M protein was performed with 817 isolates (535 GAS and 282 GCS/GGS). We also performed genotyping of the C-repeat region in 769 of these isolates to identify J14 sequence types. The profile of emm types for Fiji was very different from that found for the United States and Europe. There were no dominant emm types and a large number of overlapping types among clinical disease states. Commonly found GAS emm types in industrialized countries, including emm1, emm12, and emm28, were not found among GAS isolates from Fiji. Over 93% of GAS isolates and over 99% of GCS/GGS isolates that underwent J14 sequence typing contained either J14.0 or J14.1. Our data have implications for GAS vaccine development in developing countries and suggest that a vaccine based upon the conserved region of the M protein may be a feasible option for Fiji and potentially for other tropical developing countries.

Details

Title: emm and C-Repeat Region Molecular Typing of Beta-Hemolytic Streptococci in a Tropical Country: Implications for Vaccine Development
Authors: A C Steer (Author) - University of Melbourne
G Magor (Author) - Queensland Institute of Medical Research
A W J Jenney (Author) - University of Melbourne
J Kado (Author) - Fiji Ministry of Health, Fiji Islands
M F Good (Author) - Queensland Institute of Medical Research
David J McMillan (Author) - Queensland Institute of Medical Research
M Batzloff (Author) - Queensland Institute of Medical Research
J R Carapetis (Author) - University of Melbourne
Publication details: Journal of Clinical Microbiology, Vol.47(8), pp.2502-2509
Publisher: American Society for Microbiology
Date published: 2009
DOI: 10.1128/JCM.00312-09
ISSN: 0095-1137
Copyright note: Copyright © 2009 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450018802621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology

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