Journal article
eSnail: a transcriptome-based molecular resource of the central nervous system for terrestrial gastropods
Molecular Ecology Resources, Vol.18(1), pp.147-158
2018
Abstract
To expand on emerging terrestrial gastropod molecular resources, we have undertaken transcriptome-based sequencing of the central nervous system (CNS) from six ecologically invasive terrestrial gastropods. Focusing on snail species Cochlicella acuta and Helix aspersa, and reticulated slugs Deroceras invadens, Deroceras reticulatum, Lehmannia nyctelia, and Milax gagates, we obtained a total of 367,869,636 high quality reads and compared them with existing CNS transcript resources for the invasive Mediterranean snail, Theba pisana. In total we obtained, 419,289 unique transcripts (unigenes) from 1,410,569 assembled contigs, with BLAST search analysis of multiple protein databases leading to the annotation of 124,268 unigenes, of which 92,544 mapped to NCBI non-redundant protein databases. We found that these transcriptomes have representatives in most biological functions, based on comparison of gene ontology, KEGG pathway, and protein family contents, demonstrating a high range of transcripts responsible for regulating metabolic activities and molecular functions occurring within the CNS. To provide an accessible genetic resource, we also demonstrate the presence of 66,687 microsatellites and 304,693 single nucleotide variants, which can be used for the design of potentially thousands of unique primers for functional screening. An online 'eSnail' database with a user-friendly web interface was implemented to query all the information obtained herein (http://soft.bioinfo-minzhao.org/esnail). We demonstrate the usefulness of the database through the mining of molluscan neuropeptides. As the most comprehensive CNS transcriptome resource for terrestrial gastropods, eSnail may serve as a useful gateway for researchers to explore gastropod CNS function for multiple purposes, including for the development of biocontrol approaches.
Details
- Title
- eSnail: a transcriptome-based molecular resource of the central nervous system for terrestrial gastropods
- Authors
- Min Zhao (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTianfang Wang (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMichael J Stewart (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringUtpal Bose (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringSaowaros Suwansa-ard (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringKenneth B Storey (Author) - Charleton University, CanadaScott F Cummins (Corresponding Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Molecular Ecology Resources, Vol.18(1), pp.147-158
- Publisher
- Wiley-Blackwell Publishing Ltd.
- Date published
- 2018
- DOI
- 10.1111/1755-0998.12722
- ISSN
- 1755-098X
- Copyright note
- Copyright © 2018 Wiley-Blackwell Publishing Ltd. This is the accepted version of the following article: Zhao M, Wang T, Stewart MJ, et al. eSnail: A transcriptome-based molecular resource of the central nervous system for terrestrial gastropods. Mol Ecol Resour. 2018;18:147-158. https://doi.org/10.1111/1755-0998.12722, which has been published in final form at http://dx.doi.org/10.1111/1755-0998.12722
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99451174702621
- Output Type
- Journal article
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