Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt

Jennifer Chandler; Quinn P Fitzgibbon; Greg Smith; Abigail Elizur; Tomer Ventura

doi:10.1016/j.ydbio.2017.08.031

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Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt

Journal article

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Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt

Jennifer Chandler, Quinn P Fitzgibbon, Greg Smith, Abigail Elizur and Tomer Ventura

Developmental Biology, Vol.430(2), pp.337-345

2017

DOI: https://doi.org/10.1016/j.ydbio.2017.08.031

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Abstract

master sex-determinant

Dmrt

DMY

transactivation domain

Sex determination pathways are extensively diverse across species, with the master sex-determinants being the most variable element. Despite this, there is a family of DM-domain transcription factors (Dmrts), which hold a highly conserved function in sexual development. This work is the first to describe a heterogametic sex-linked Dmrt in an invertebrate species, the Eastern spiny lobster, Sagmariasus verreauxi. We have termed the Y-linked, truncated paralogue of the autosomal iDmrt1, Sv-iDMY. Considering the master sex-determining function of both DMY in medaka and DM-W in frog, we hypothesised a similar function of Sv-iDMY. By conducting temporal expression analyses during embryogenesis we have identified a putative male sex-determining period during which iDMY > iDmrt1. Employing a GAL4-transactivation assay we then demonstrate the dominant negative suppression of iDMY over its autosomal iDmrt1 paralogue, suggesting the mechanism with which iDMY determines sex. Comparative analyses of Sv-iDMY, DM-W and medaka DMY, highlight the C'-mediated features of oligomerisation and transactivation as central to the mechanism that each exerts. Indeed, these features may underpin the plasticity facilitating the convergent emergence of these three sporadic sex-linked master-Dmrts.

Details

Title: Y-linked iDmrt1 paralogue (iDMY) in the Eastern spiny lobster, Sagmariasus verreauxi: the first invertebrate sex-linked Dmrt
Authors: Jennifer Chandler (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Quinn P Fitzgibbon (Author) - University of Tasmania
Greg Smith (Author) - University of Tasmania
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Tomer Ventura (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Developmental Biology, Vol.430(2), pp.337-345
Publisher: Academic Press
Date published: 2017
DOI: 10.1016/j.ydbio.2017.08.031
ISSN: 0012-1606
Grants: Redefining the molecular mechanism underlying crustacean metamorphosis, DP160103320, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450322802621
Output Type: Journal article

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