Wood Species Differentiation: A Comparative Study of Direct Analysis in Real-Time and Chromatography Mass Spectrometry

Ilena Isak; Harriet Laura Newson; Tripti Singh

doi:10.3390/f16020255

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Wood Species Differentiation: A Comparative Study of Direct Analysis in Real-Time and Chromatography Mass Spectrometry

Journal article

Open access

Peer reviewed

Wood Species Differentiation: A Comparative Study of Direct Analysis in Real-Time and Chromatography Mass Spectrometry

Ilena Isak, Harriet Laura Newson and Tripti Singh

Forests, Vol.16(2), pp.1-14

2025

DOI: https://doi.org/10.3390/f16020255

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Abstract

DART-TOF-MS

wood species differentiation

chemometric analysis

This study reports for the first time the fingerprinting extractives analysis of the indigenous wood species of Podocarpus totara from New Zealand, Eucalyptus saligna from Australia and Pinus radiata imported from California, USA and grown in New Zealand. We evaluated the use of analytical techniques for wood species discrimination. We compared the chemical fingerprinting of extractive compounds obtained using traditional chromatographic techniques with direct analysis in real-time–time of flight-mass spectrometry (DART-TOF-MS) with the auxiliary of chemometrics and principal component analysis. The traditional wet chemistry analysis of wood extracts provided a comprehensive characterisation of all extractive components. However, the more eco-friendly, sustainable and faster DART-TOF-MS technique effectively distinguished between wood species when heartwood and sapwood samples were combined. Notably, neither wet chemistry nor DART-TOF-MS could clearly differentiate between heartwood and sapwood within the same wood species. DART-TOF-MS analysis demonstrates potential as a reliable quality control tool for identifying wood species necessary in commercial and timber trading markets as well as for detecting the illicit trade of counterfeit wood products.

Details

Title: Wood Species Differentiation: A Comparative Study of Direct Analysis in Real-Time and Chromatography Mass Spectrometry
Authors: Ilena Isak (Corresponding Author) - Scion
Harriet Laura Newson - ChemCentre
Tripti Singh - University of the Sunshine Coast, Queensland, National Centre for Timber Durability and Design Life
Publication details: Forests, Vol.16(2), pp.1-14
Publisher: MDPI AG
Date published: 2025
DOI: 10.3390/f16020255
ISSN: 1999-4907
Copyright note: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)
Data Availability: The original contributions presented in the study are included in the article and Supplementary Materials. Further inquiries can be directed to the corresponding author.
Grant note: This research was supported by funding from New Zealand Ministry of Business Innovation and Employment through the Strategic Science Investment Fund—Manufactured Products From Trees science platform contract number C04X1703.
Organisation Unit: National Centre for Timber Durability and Design Life
Language: English
Record Identifier: 991102145402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Forestry