Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients

Linda T Viberg; Derek S Sarovich; Timothy J Kidd; James B Geake; Scott C Bell; Bart J Currie; Erin P Price

doi:10.1128/mBio.00356-17

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Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients

Journal article

Open access

Peer reviewed

Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients

Linda T Viberg, Derek S Sarovich, Timothy J Kidd, James B Geake, Scott C Bell, Bart J Currie and Erin P Price

mBio, Vol.8(2), e00356-17

2017

DOI: https://doi.org/10.1128/mBio.00356-17

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Abstract

Burkholderia pseudomallei

antibiotic resistance

cystic fibrosis

melioidosis

parallelism

reductive evolution

whole-genome sequencing

within-host evolution

Cystic fibrosis (CF) is a genetic disorder characterized by progressive lung function decline. CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia pseudomallei, the causative agent of melioidosis. Here, we compared the genomes of B. pseudomallei isolates collected between ~4 and 55 months apart from seven chronically infected CF patients. Overall, the B. pseudomallei strains showed evolutionary patterns similar to those of other chronic infections, including emergence of antibiotic resistance, genome reduction, and deleterious mutations in genes involved in virulence, metabolism, environmental survival, and cell wall components. We documented the first reported B. pseudomallei hypermutators, which were likely caused by defective MutS. Further, our study identified both known and novel molecular mechanisms conferring resistance to three of the five clinically important antibiotics for melioidosis treatment. Our report highlights the exquisite adaptability of microorganisms to long-term persistence in their environment and the ongoing challenges of antibiotic treatment in eradicating pathogens in the CF lung. Convergent evolution with other CF pathogens hints at a degree of predictability in bacterial evolution in the CF lung and potential targeted eradication of chronic CF infections in the future.

Details

Title: Within-Host Evolution of Burkholderia pseudomallei during Chronic Infection of Seven Australasian Cystic Fibrosis Patients
Authors: Linda T Viberg (Author) - Charles Darwin University
Derek S Sarovich (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Timothy J Kidd (Author) - University of Queensland
James B Geake (Author) - The Lyell McEwin Hospital
Scott C Bell (Author) - QIMR Berghofer Medical Research Institute
Bart J Currie (Author) - Charles Darwin University
Erin P Price (Corresponding Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: mBio, Vol.8(2), e00356-17; 16
Publisher: American Society for Microbiology
Date published: 2017
DOI: 10.1128/mBio.00356-17
ISSN: 2150-7511
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99451142302621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology

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