Whole genome shotgun sequences for microsatellite discovery and application in cultivated and wild Macadamia (Proteaceae)

Catherine J. Nock; Martin S. Elphinstone; Gary Ablett; Asuka Kawamata; Wayne Hancock; Craig M. Hardner; Graham J. King

doi:10.3732/apps.1300089

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Whole genome shotgun sequences for microsatellite discovery and application in cultivated and wild Macadamia (Proteaceae)

Journal article

Open access

Peer reviewed

Whole genome shotgun sequences for microsatellite discovery and application in cultivated and wild Macadamia (Proteaceae)

Catherine J. Nock, Martin S. Elphinstone, Gary Ablett, Asuka Kawamata, Wayne Hancock, Craig M. Hardner and Graham J. King

Applications in Plant Sciences, Vol.2(4), pp.1-4

2014

DOI: https://doi.org/10.3732/apps.1300089

PMID: 25202615

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Abstract

crop

cultivar

horticulture

Macadamia

nut

Proteaceae

• Premise of the study: Next-generation sequencing (NGS) data are widely used for single-nucleotide polymorphism discovery and genetic marker development in species with limited available genome information. We developed microsatellite primers for the Proteaceae nut crop species Macadamia integrifolia and assessed cross-species transferability in all congeners to investigate genetic identification of cultivars and gene flow. • Methods and Results: Primers were designed from both raw and assembled Illumina NGS paired-end reads. The final 12 microsatellite markers selected were polymorphic among wild individuals of all four Macadamia species—M. integrifolia, M. tetraphylla, M. ternifolia, and M. jansenii—and in commercial macadamia cultivars including hybrids. • Conclusions: We demonstrate the utility of raw and assembled Illumina NGS reads from total genomic DNA for the rapid development of microsatellites in Macadamia. These primers will facilitate future studies of population structure, hybridization, parentage, and cultivar identification in cultivated and wild Macadamia populations.

Details

Title: Whole genome shotgun sequences for microsatellite discovery and application in cultivated and wild Macadamia (Proteaceae)
Authors: Catherine J. Nock (Corresponding Author) - Southern Cross University
Martin S. Elphinstone - Southern Cross University
Gary Ablett - Southern Cross University
Asuka Kawamata - Southern Cross University
Wayne Hancock - Southern Cross University
Craig M. Hardner - The University of Queensland
Graham J. King - Southern Cross University
Publication details: Applications in Plant Sciences, Vol.2(4), pp.1-4
Publisher: John Wiley & Sons Ltd.
Date published: 2014
DOI: 10.3732/apps.1300089
ISSN: 2168-0450
PMID: 25202615
Copyright note: © 2014 The Author(s) This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
Organisation Unit: Australian Centre for Pacific Islands Research
Language: English
Record Identifier: 991007532702621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Plant Sciences