Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island

Derek S Sarovich; Stephanie N J Chapple; Erin P Price; Mark Mayo; Matthew T G Holden; Sharon J Peacock; Bart J Currie

doi:10.1099/mgen.0.000117

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Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island

Journal article

Open access

Peer reviewed

Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island

Derek S Sarovich, Stephanie N J Chapple, Erin P Price, Mark Mayo, Matthew T G Holden, Sharon J Peacock and Bart J Currie

Microbial genomics, Vol.3

2017

DOI: https://doi.org/10.1099/mgen.0.000117

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Abstract

melioidosis

source tracing

Burkholderia pseudomallei

recombination

population genetics

outbreak

Melioidosis is a tropical disease caused by the bacterium Burkholderia pseudomallei. Outbreaks are uncommon and can generally be attributed to a single point source and strain. We used whole-genome sequencing to analyse B. pseudomallei isolates collected from an historical 2-year long case cluster that occurred in a remote northern Australian indigenous island community, where infections were previously linked to a contaminated communal water supply. We analysed the genome-wide relatedness of the two most common multilocus sequence types (STs) involved in the outbreak, STs 125 and 126. This analysis showed that although these STs were closely related on a whole-genome level, they demonstrated evidence of multiple recombination events that were unlikely to have occurred over the timeframe of the outbreak. Based on epidemiological and genetic data, we also identified two additional patients not previously associated with this outbreak. Our results confirm the previous hypothesis that a single unchlorinated water source harbouring multiple B. pseudomallei strains was linked to the outbreak, and that increased melioidosis risk in this community was associated with Piper methysticum root (kava) consumption.

Details

Title: Whole-genome sequencing to investigate a non-clonal melioidosis cluster on a remote Australian island
Authors: Derek S Sarovich (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Stephanie N J Chapple (Author) - Menzies School of Health Research
Erin P Price (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Mark Mayo (Author) - Menzies School of Health Research
Matthew T G Holden (Author) - University of St Andrews, United Kingdom
Sharon J Peacock (Author) - University of Cambridge, United Kingdom
Bart J Currie (Author) - Menzies School of Health Research
Publication details: Microbial genomics, Vol.3; 7
Publisher: Microbiology Society
Date published: 2017
DOI: 10.1099/mgen.0.000117
ISSN: 2057-5858
Copyright note: Copyright © 2017 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99451288502621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Genetics & Heredity; Microbiology

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