Virulence characteristics of translocating Escherichia coli and the interleukin-8 response to infection

Nubia Ramos; A Lamprokostopoulou; T A Chapman; J C Chin; U Romling; A Brauner; Mohammad Katouli

doi:10.1016/j.micpath.2010.11.003

Back

Virulence characteristics of translocating Escherichia coli and the interleukin-8 response to infection

Journal article

Peer reviewed

Virulence characteristics of translocating Escherichia coli and the interleukin-8 response to infection

Nubia Ramos, A Lamprokostopoulou, T A Chapman, J C Chin, U Romling, A Brauner and Mohammad Katouli

Microbial Pathogenesis, Vol.50(2), pp.81-86

2011

DOI: https://doi.org/10.1016/j.micpath.2010.11.003

Files and links (1)

url

https://doi.org/10.1016/j.micpath.2010.11.003View

Published Version

Abstract

E.coli

translocation

virulence genes

IL-8 production

Four efficiently translocating E. coli (TEC) strains isolated from the blood of humans (HMLN-1), pigs (PC-1) and rats (KIC-1 and KIC-2) were tested for their ability to adhere and translocate across human gut epithelial Caco-2 and HT-29 cells, to elicit a proinflammatory response and for the presence of 47 pathogenic E. coli virulence genes. HMLN-1 and PC-1 were more efficient in adhesion and translocation than rat strains, had identical biochemical phenotype (BPT) and serotype (O77:H18) and phylogenetic group (D). KIC-2 adhered more than KIC-1, belonged to different BPT and serotype but the same phylogenetic group as KIC-1. TEC strains elicited significantly higher IL-8 response in both cell lines (P < 0.05) and monocytic THP-1 (P < 0.0001) cells than non-TEC strains. KIC-2 induced the highest IL-8 response which may be associated with its immunostimulatory flagellin. Apart from adhesin genes fimH and bmaE that were carried by all strains, HMLN-1 and PC-1 carried capsule synthesis gene kpsMT III and KIC-2 carried the EAST1 toxin gene. The lack of known virulence genes and the ability of TEC to efficiently adhere and translocate whilst causing proinflammatory response suggests that these strains may carry as yet unidentified genes that enable their translocating ability.

Details

Title: Virulence characteristics of translocating Escherichia coli and the interleukin-8 response to infection
Authors: Nubia Ramos (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
A Lamprokostopoulou (Author) - Karolinska Institutet, Sweden
T A Chapman (Author) - New South Wales Department of Primary Industry
J C Chin (Author) - New South Wales Department of Primary Industry
U Romling (Author) - Karolinska Institutet, Sweden
A Brauner (Author) - Karolinska Institutet, Sweden
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Publication details: Microbial Pathogenesis, Vol.50(2), pp.81-86
Publisher: Academic Press
Date published: 2011
DOI: 10.1016/j.micpath.2010.11.003
ISSN: 0882-4010
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450156102621
Output Type: Journal article

Metrics

2 File views/ downloads

517 Record Views

5 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Immunology; Microbiology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites