Viability and stability of Escherichia coli and enterococci populations in fecal samples upon freezing

Nicole Masters; Melodie Christie; Helen Stratton; Mohammad Katouli

doi:10.1139/cjm-2015-0020

Back

Viability and stability of Escherichia coli and enterococci populations in fecal samples upon freezing

Journal article

Peer reviewed

Viability and stability of Escherichia coli and enterococci populations in fecal samples upon freezing

Nicole Masters, Melodie Christie, Helen Stratton and Mohammad Katouli

Canadian Journal of Microbiology, Vol.61(7), pp.495-501

2015

DOI: https://doi.org/10.1139/cjm-2015-0020

Files and links (1)

url

https://doi.org/10.1139/cjm-2015-0020View

Published Version

Abstract

deep freezing

PhP typing

bacterial composition

diversity

population structure

We studied the survival of Escherichia coli and enterococci populations in faecal samples of seven host species after storage at -20°C and -80°C for 30 days. The numbers of E. coli and enterococci in composite faecal samples of cows, chickens, horses, pigs, dogs, birds and humans were enumerated before and after storage. From each composite sample, 28 colonies of each bacterial species were typed before and after storage and the strains were assigned to different biochemical phenotypes (BPTs). A significant reduction in the number of E. coli was observed in all samples stored at -20°C, and three of the seven animal samples stored at -80°C. However, no difference in the number of enterococci was found in all stored samples (except cow and birds). The number and the distribution of E. coli and enterococci BPTs in fresh samples did not vary significantly from those stored at either temperature. Short term storage of faecal samples at -80°C does not significantly change the population structure of E. coli and enterococci populations. This study provides a viable option for storing faecal samples when resources limit immediate microbiological analyses.

Details

Title: Viability and stability of Escherichia coli and enterococci populations in fecal samples upon freezing
Authors: Nicole Masters (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Melodie Christie (Author) - Griffith University
Helen Stratton (Author) - Griffith University
Mohammad Katouli (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Publication details: Canadian Journal of Microbiology, Vol.61(7), pp.495-501
Publisher: N R C Research Press
Date published: 2015
DOI: 10.1139/cjm-2015-0020
ISSN: 0008-4166
Organisation Unit: School of Health; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99449182502621
Output Type: Journal article

Metrics

20 File views/ downloads

772 Record Views

11 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Web Of Science research areas: Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Immunology; Microbiology

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites