Journal article
Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease
Atherosclerosis, Vol.246, pp.98-105
2016
Abstract
Background: Peripheral arterial disease (PAD) is characterised by impaired leg blood flow, which contributes to claudication and reduced exercise capacity. This study investigated to what extent vasoactive enzymes might contribute to altered blood flow in PAD (Fontaine stage II). Methods: We compared femoral artery blood flow during reactive hyperaemia, leg-extension exercise and passive leg movement, and determined the level of vasoactive enzymes in skeletal muscle samples from the vastus lateralis in PAD (n = 10, 68.5±6.5 years) and healthy controls (CON, n = 9, 62.1±12.3 years). Leg blood flow was measured with Doppler ultrasound and muscle protein levels of phosphorylated endothelial nitric oxide synthase, NADPH oxidase, cyclooxygenase 1 and 2, thromboxane synthase, and prostacyclin synthase were determined. Results: Leg blood flow during the initial 90s of passive leg movement (242±33 vs 441±75 ml∙min-1, P = 0.03) and during reactive hyperaemia (423±100 vs 1255±175 ml∙min-1, P = 0.002) was lower in PAD than CON, whereas no significant difference was observed for leg blood flow during exercise (1490±250 vs 1887±349 ml∙min-1, P = 0.37). PAD had higher NADPH oxidase than CON (1.04±0.19 vs 0.50±0.06 AU, P = 0.02), with no differences for other enzymes. Leg blood flow during exercise was correlated with prostacyclin synthase (P = 0.001). Conclusion: Elevated NADPH oxidase indicates that oxidative stress may be a primary cause of low nitric oxide availability and impaired blood flow in PAD.
Details
- Title
- Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease
- Authors
- Meegan A Walker (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringBirgitte Hoier (Author) - University of Copenhagen, DenmarkPhilip J Walker (Author) - University of QueenslandKarl Schulze (Author) - Sunshine Vascular Surgery and ImagingJens Bangsbo (Author) - University of Copenhagen, DenmarkYlva Hellsten (Author) - University of Copenhagen, DenmarkChristopher D Askew (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Atherosclerosis, Vol.246, pp.98-105
- Publisher
- Elsevier Ireland Ltd.
- Date published
- 2016
- DOI
- 10.1016/j.atherosclerosis.2015.12.029
- ISSN
- 0021-9150
- Copyright note
- Copyright © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Health and Sport Sciences - Legacy; School of Health - Sports & Exercise Science; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99450056002621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Cardiac & Cardiovascular Systems
- Peripheral Vascular Disease
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Source: InCites