Variations in biochemical phenotypes and phage types of Salmonella enteriditis in Germany 1980-1982

Mohammad Katouli; R Seuffer; R Wollin; I Kühn; R Möllby

doi:10.1017/S0950268800056909

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Variations in biochemical phenotypes and phage types of Salmonella enteriditis in Germany 1980-1982

Journal article

Peer reviewed

Variations in biochemical phenotypes and phage types of Salmonella enteriditis in Germany 1980-1982

Mohammad Katouli, R Seuffer, R Wollin, I Kühn and R Möllby

Epidemiology and Infection, Vol.111(2), pp.199-207

1993

DOI: https://doi.org/10.1017/S0950268800056909

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Abstract

Medical and Health Sciences

Biological Sciences

Agricultural and Veterinary Sciences

The Phene Plate system for typing Salmonella serotypes (PhP-S) is a simple automated typing method based on biochemical fingerprinting. It gives a quantitative value of the metabolism of various substrates by measuring the speed and intensity of each reaction. The 'biochemical fingerprint' of each isolate is used to calculate similarities among the tested strains with a personal computer program. We used this system to examine a collection of 86 strains of Salmonella enteritidis isolated from human sporadic cases in Germany between 1980 and 1992. Twenty-three biochemical phenotypes (BPTs) consisting of 9 common (C) and 14 single (S) BPTs were identified. BPTs C2 and C4 containing 20 and 36 strains respectively accounted for 65% of the isolates. Strains of BPT C2 were found over a wide period of time whereas strains of BPT C4 were isolated during the period between 1988 and 1992. With phage typing, 11 discrete phage types (PTs) and 18 strains designated as non-specific type (NST) were identified. PTs 4 and 8 with 39 and 17 strains respectively were the dominant PTs. Strains of PT 8 were isolated over a wide period of time whereas all (except one) strains of PT 4 were isolated between 1988 and 1992. Combination of biochemical fingerprinting and phage typing divided the strains into 25 phenotypes (BPT:PTs). Whilst phenotype C2:8 was found over a number of different years, phenotype C4:4 was isolated only between 1988 and 1992. These findings indicate the presence of one persistent and one recently emerged phenotype among S. enteritidis strains in Germany. Although both methods identified the presence of both major and less common types, biochemical fingerprinting with the PhP-S system also provided information about relationships between the strains.

Details

Title: Variations in biochemical phenotypes and phage types of Salmonella enteriditis in Germany 1980-1982
Authors: Mohammad Katouli (Author) - Karolinska Institute, Sweden
R Seuffer (Author) - Laboratory for Microbiology and Epidemiology, Germany
R Wollin (Author) - National Bacteriological Laboratory, Sweden
I Kühn (Author) - Karolinska Institute, Sweden
R Möllby (Author) - Karolinska Institute, Sweden
Publication details: Epidemiology and Infection, Vol.111(2), pp.199-207
Publisher: Cambridge University Press
Date published: 1993
DOI: 10.1017/S0950268800056909
ISSN: 0950-2688
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450140802621
Output Type: Journal article

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