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Variants of human papillomaviruses 16 (HPV16) in Uigur women in Xinjiang, China
Journal article   Open access   Peer reviewed

Variants of human papillomaviruses 16 (HPV16) in Uigur women in Xinjiang, China

Hongchang He, Hongtao Li, Peiwen Fan, Junling Zhu, Zhenzhen Pan, Huan Pan, Dan Wu, Xianxian Ren, Xiaoqing Guo, Dongmei Li, …
Infectious Agents and Cancer, Vol.11, 44
2016
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https://doi.org/10.1186/s13027-016-0089-2View
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Abstract

cervical cancer HPV16 Uigur women
Background: Persistent infection of high-risk human papillomaviruses 16 (HPV16) has been considered as the leading cause of cervical cancer. In this study we assessed HPV16 sequence variation and genetic diversity of HPV16 variants in cervical cancer in Uigur women in Xinjiang, China. We analyzed the nucleotide sequences of the open reading frames of E6 and E7, and part of the open reading frames of L1 of HPV16 in Uigur women. Methods: Biopsies of histologically confirmed HPV16 infections with cervical cancer were obtained from 43 Uigur women in Xinjiang, China. E6, E7 and L1 genes of HPV16 of all samples were amplified and sequenced; the sequences were used in phylogenetic analysis of HPV16 variants. Results: Our analysis revealed nine nucleotide changes in E6 (five changes), E7 (one change) and L1 (three changes) gene. The most frequently observed variations were T350G (79.1 %). One variation T295G (D64E) at E6 were detected in 6 cases (KT959536, KT959542, KT959546, KT959550, KT959553, KT959558). Deletion (464Asp) along with insertion (448Ser) were observed in L1 (100 %). Most variants were European lineage (97.7 %); only one belongs to Asia variants with common T178G (D25E) in E6 and A647G (N29S) in E7. Conclusion: The most prevalent HPV16 variants in the Uigur women we studied were of the European lineage. Our results indicate that HPV16 European lineage may serve as a harmful factor associated with the development and progression of cervical cancer.

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