Journal article
Variability in the Distribution of Genes Encoding Virulence Factors and Putative Extracellular Proteins of Streptococcus pyogenes in India, a Region with High Streptococcal Disease Burden, and Implication for Development of a Regional Multisubunit Vaccine
Clinical and Vaccine Immunology, Vol.19(11), pp.1818-1825
2012
Abstract
Streptococcus pyogenes causes a wide variety of human diseases and is a significant cause of morbidity and mortality. Attempts to develop a vaccine were hampered by the genetic diversity of S. pyogenes across different regions of the world. This study sought to identify streptococcal antigens suitable for a region-specific vaccine in India. We used a two-step approach, first performing epidemiological analysis to identify the conserved antigens among Indian isolates. The second step consisted of validating the identified antigens by serological analysis. The 201 streptococcal clinical isolates from India used in this study represented 69 different emm types, with emm12 being the most prevalent. Virulence profiling of the North and South Indian S. pyogenes isolates with a custom-designed streptococcal virulence microarray identified seven conserved putative vaccine candidates. Collagen-like surface protein (SCI), putative secreted 5′-nucleotidase (PSNT), and C5a peptidase were found in 100% of the isolates, while R28, a putative surface antigen (PSA), and a hypothetical protein (HYP) were found in 90% of the isolates. A fibronectin binding protein, SfbI, was present in only 78% of the isolates. In order to validate the identified potential vaccine candidates, 185 serum samples obtained from patients with different clinical manifestations were tested for antibodies. Irrespective of clinical manifestations, serum samples showed high antibody titers to all proteins except for SCI and R28. Thus, the data indicate that PSNT, C5a peptidase, PSA, HYP, and SfbI are promising candidates for a region-specific streptococcal vaccine for the different parts of India.
Details
- Title
- Variability in the Distribution of Genes Encoding Virulence Factors and Putative Extracellular Proteins of Streptococcus pyogenes in India, a Region with High Streptococcal Disease Burden, and Implication for Development of a Regional Multisubunit Vaccine
- Authors
- V Sagar (Author) - Helmholtz Centre for Infection Research, GermanyR Bergmann (Author) - Helmholtz Centre for Infection Research, GermanyA Nerlich (Author) - Helmholtz Centre for Infection Research, GermanyDavid J McMillan (Author) - Queensland Institute of Medical ResearchP Nitsche Schmitz (Author) - Helmholtz Centre for Infection Research, GermanyG S Chhatwal (Author) - Helmholtz Centre for Infection Research, Germany
- Publication details
- Clinical and Vaccine Immunology, Vol.19(11), pp.1818-1825
- Publisher
- American Society for Microbiology
- Date published
- 2012
- DOI
- 10.1128/CVI.00112-12
- ISSN
- 1556-6811
- Copyright note
- Copyright © 2012 American Society for Microbiology. Reproduced here in accordance with the publishers copyright policy.
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99450225602621
- Output Type
- Journal article
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- Immunology
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- Microbiology
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