Files and links (2)
Published VersionCC BY V4.0, Open Access
Journal article
Validation of a Microsphere Immunoassay for Serological Leptospirosis Diagnosis in Human Serum by Comparison to the Current Gold Standard
PLoS Neglected Tropical Diseases, Vol.9(3), pp.1-13
2015
Abstract
A microsphere immunoassay (MIA) utilising Luminex xMap technology that is capable of determining leptospirosis IgG and IgM independently was developed. The MIA was validated using 200 human samples submitted for routine leptospirosis serology testing. The traditional microscopic agglutination (MAT) method (now 100 years old) suffers from a significant range of technical problems including a dependence on antisera which is difficult to source and produce, false positive reactions due to auto-agglutination and an inability to differentiate between IgG and IgM antibodies. A comparative validation method of the MIA against the MAT was performed and used to determine the ability of the MIA to detect leptospiral antibodies when compared with the MAT. The assay was able to determine samples in the reactive, equivocal and non-reactive ranges when compared to the MAT and was able to differentiate leptospiral IgG antibodies from leptospiral IgM antibodies. The MIA is more sensitive than the MAT and in true infections was able to detect low levels of antibody in the later stages of the acute phase as well as detect higher levels of IgM antibody earlier in the immune phase of the infection. The relatively low cost, high throughput platform and significantly reduced dependency on large volumes of rabbit antisera make this assay worthy of consideration for any microbiological assay that currently uses agglutination assays.
Details
- Title
- Validation of a Microsphere Immunoassay for Serological Leptospirosis Diagnosis in Human Serum by Comparison to the Current Gold Standard
- Authors
- Sarah Jane Wynwood (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringMary-Anne A Burns (Author) - Queensland Health Forensic and Scientific ServicesGlenn C Graham (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringSteven L Weier (Author) - Queensland University of TechnologyDavid B McKay (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringScott B Craig (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- PLoS Neglected Tropical Diseases, Vol.9(3), pp.1-13
- Publisher
- Public Library of Science
- Date published
- 2015
- DOI
- 10.1371/journal.pntd.0003636
- ISSN
- 1935-2727; 1935-2727
- Copyright note
- Copyright © 2015 Wynwood et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Organisation Unit
- School of Science and Engineering - Legacy; School of Science, Technology and Engineering
- Language
- English
- Record Identifier
- 99449402602621
- Output Type
- Journal article
Metrics
76 File views/ downloads
1097 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Web Of Science research areas
- Infectious Diseases
- Parasitology
- Tropical Medicine
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites