Vaccination of healthy and diseased koalas (Phascolarctos cinereus) with a Chlamydia pecorum multi-subunit vaccine: Evaluation of immunity and pathology

A Kollipara; C George; Jon Hanger; J Loader; Adam Polkinghorne; K W Beagley; Peter Timms

doi:10.1016/j.vaccine.2011.12.125

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Vaccination of healthy and diseased koalas (Phascolarctos cinereus) with a Chlamydia pecorum multi-subunit vaccine: Evaluation of immunity and pathology

Journal article

Open access

Peer reviewed

Vaccination of healthy and diseased koalas (Phascolarctos cinereus) with a Chlamydia pecorum multi-subunit vaccine: Evaluation of immunity and pathology

A Kollipara, C George, Jon Hanger, J Loader, Adam Polkinghorne, K W Beagley and Peter Timms

Vaccine, Vol.30(10), pp.1875-1885

2012

DOI: https://doi.org/10.1016/j.vaccine.2011.12.125

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Abstract

Chlamydia

Koala

vaccine

antibody

Chlamydial infections represent a major threat to the long-term survival of the koala and a successful vaccine would provide a valuable management tool. Vaccination however has the potential to enhance inflammatory disease in animals exposed to a natural infection prior to vaccination, a finding in early human and primate trials of whole cell vaccines to prevent trachoma. In the present study, we vaccinated both healthy koalas as well as clinically diseased koalas with a multi-subunit vaccine consisting of Chlamydia pecorum MOMP and NrdB mixed with immune stimulating complex as adjuvant. Following vaccination, there was no increase in inflammatory pathological changes in animals previously infected with Chlamydia. Strong antibody (including neutralizing antibodies) and lymphocyte proliferation responses were recorded in all vaccinated koalas, both healthy and clinically diseased. Vaccine induced antibodies specific for both vaccine antigens were observed not only in plasma but also in ocular secretions. Our data shows that an experimental chlamydial vaccine is safe to use in previously infected koalas, in that it does not worsen infection-associated lesions. Furthermore, the prototype vaccine is effective, as demonstrated by strong levels of neutralizing antibody and lymphocyte proliferation responses in both healthy and clinically diseased koalas. Collectively, this work illustrates the feasibility of developing a safe and effective Chlamydia vaccine as a tool for management of disease in wild koalas. © 2012 Elsevier Ltd.

Details

Title: Vaccination of healthy and diseased koalas (Phascolarctos cinereus) with a Chlamydia pecorum multi-subunit vaccine: Evaluation of immunity and pathology
Authors: A Kollipara (Author) - Queensland University of Technology
C George (Author) - Queensland University of Technology
Jon Hanger (Author) - Australia Zoo Wildlife Hospital
J Loader (Author) - Queensland University of Technology
Adam Polkinghorne (Author) - Queensland University of Technology
K W Beagley (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: Vaccine, Vol.30(10), pp.1875-1885
Publisher: Elsevier Ltd.
Date published: 2012
DOI: 10.1016/j.vaccine.2011.12.125
ISSN: 0264-410X
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99448812702621
Output Type: Journal article

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