Journal article
Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE
Acta Tropica, Vol.138, pp.78-82
2014
Abstract
An approach to improve the diagnosis of Strongyloides stercoralis infection is the use of serologic assays utilising the NIE antigen from S. stercoralis, with good diagnostic sensitivity and excellent specificity reported. Detection of antibody eluted from dried blood spots (DBS) has shown utility in large-scale seroepidemiological studies for a range of conditions and is appealing for use with children where sample collection is difficult. We adapted an existing NIE-enzyme linked immunosorbent assay (ELISA) for the testing of strongyloides antibody response on DBS, and evaluated it in a population screening and mass drug administration programme (MDA) for strongyloidiasis conducted in an Australian indigenous community. Study participants were treated with 200 μg/kg ivermectin (>15 kg) or 3× 400 mg albendazole (less than 15 kg). The sensitivity of the NIE DBS-ELISA was determined by receiver operator characteristic (ROC) analysis to be 85.7%. A total of 214 DBS were collected from 184 participants across two screening and MDA encounters. A total of 27 of 164 participants (16.5%) tested positive for S. stercoralis NIE-DBS prior to MDA treatment, and 6 of 50 participants (12.0%) tested positive after treatment. These prevalence values are similar to those documented by standard serology in the same community. For 30 participants where a DBS was collected at both MDA 1 and 2, a significant decline in ELISA values was evident post treatment (0.12-0.02, p = 0.0012). These results are in agreement with previous studies documenting the high seroprevalence of S. stercoralis in remote Australian Indigenous communities, and suggest that collection of dried blood spots may be a useful approach for field diagnosis of S. stercoralis seroprevalence.
Details
- Title
- Use of dried blood spots to define antibody response to the Strongyloides stercoralis recombinant antigen NIE
- Authors
- Kate E Mounsey (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringTherese Kearns (Author) - Charles Darwin UniversityMelanie Rampton (Author) - QIMR Berghofer Medical Research InstituteStacey Llewellyn (Author) - QIMR Berghofer Medical Research InstituteMallory King (Author) - QIMR Berghofer Medical Research InstituteDeborah Holt (Author) - Charles Darwin UniversityBart J Currie (Author) - Charles Darwin UniversityRoss Andrews (Author) - Charles Darwin UniversityThomas Nutman (Author) - National Institutes of Health, United StatesJames McCarthy (Author) - University of Queensland
- Publication details
- Acta Tropica, Vol.138, pp.78-82
- Publisher
- Elsevier BV
- Date published
- 2014
- DOI
- 10.1016/j.actatropica.2014.07.007
- ISSN
- 0001-706X
- Copyright note
- Copyright © 2014. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
- Organisation Unit
- School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
- Language
- English
- Record Identifier
- 99449060902621
- Output Type
- Journal article
Metrics
60 File views/ downloads
1208 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Parasitology
- Tropical Medicine
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites