Use of Bacteriophages to Control Vibrio Contamination of Microalgae Used as a Food Source for Oyster Larvae During Hatchery Culture

Tuan Son Le; Paul C Southgate; Wayne A O'Connor; Tomer Abramov; Daniel E Shelley; Van Sang Vu; D Ipek Kurtboke

doi:10.1007/s00284-020-01981-w

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Use of Bacteriophages to Control Vibrio Contamination of Microalgae Used as a Food Source for Oyster Larvae During Hatchery Culture

Journal article

Peer reviewed

Use of Bacteriophages to Control Vibrio Contamination of Microalgae Used as a Food Source for Oyster Larvae During Hatchery Culture

Tuan Son Le, Paul C Southgate, Wayne A O'Connor, Tomer Abramov, Daniel E Shelley, Van Sang Vu and D Ipek Kurtboke

Current Microbiology, Vol.77, pp.1811-1820

2020

DOI: https://doi.org/10.1007/s00284-020-01981-w

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Abstract

Cultured microalgae are the primary food source for oyster larvae during hatchery culture and are a potential vector for Vibrio spp. infection of larval cultures. Bacteriophages have shown potential for controlling contamination of Vibrio spp. in aquaculture systems and their application could be an effective biological control method to eliminate such bacterial contamination of microalgae. This study investigated whether Vibrio-free microalgae sources could be ensured via the application of Vibrio specific phages. As a first step, four different Vibrio bacteriophages (belonging to the Myoviridae viral family) were isolated from marine waters in Queensland, Australia and used in challenge tests against a Vibrio host species, previously isolated from New South Wales oyster hatchery and found to be closely related to V. alginolyticus (ATCC 17749). The genome sequence of one of the four isolated bacteriophages, Vibrio Φ-2, that displayed strongest virulence against the host was determined. The 242446 bp genome of this bacteriophage was predicted to encode 217 proteins with an average GC content of 43.91%, containing putative thymidine kinases and a lysin enzyme. Application of these bacteriophages to pathogenic Vibrio spp. contaminating microalgae suspensions resulted in significant decreases in their numbers within 2 h. Findings indicated that direct application of bacteriophages to microalgae suspensions could be an effective method of reducing the occurrence of vibriosis in oyster hatcheries.

Details

Title: Use of Bacteriophages to Control Vibrio Contamination of Microalgae Used as a Food Source for Oyster Larvae During Hatchery Culture
Authors: Tuan Son Le (Author) - University of the Sunshine Coast - School of Science and Engineering
Paul C Southgate (Author) - University of the Sunshine Coast - School of Science and Engineering
Wayne A O'Connor (Author) - New South Wales Fisheries
Tomer Abramov (Author) - University of the Sunshine Coast - School of Science and Engineering
Daniel E Shelley (Author) - University of the Sunshine Coast - School of Science and Engineering
Van Sang Vu (Author) - University of the Sunshine Coast - School of Science and Engineering
D Ipek Kurtboke (Author) - University of the Sunshine Coast - School of Science and Engineering
Publication details: Current Microbiology, Vol.77, pp.1811-1820
Publisher: Springer New York LLC
Date published: 2020
DOI: 10.1007/s00284-020-01981-w
ISSN: 0343-8651
Organisation Unit: School of Science and Engineering - Legacy; Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy; Technical and Work Integrated Learning (WIL) Operations - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450817502621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology