Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study

David J McMillan; P A Drèze; T Vu; D E Bessen; J Guglielmini; A C Steer; J R Carapetis; L Van Melderen; K S Sriprakash; P R Smeesters

doi:10.1111/1469-0691.12134

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Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study

Journal article

Open access

Peer reviewed

Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study

David J McMillan, P A Drèze, T Vu, D E Bessen, J Guglielmini, A C Steer, J R Carapetis, L Van Melderen, K S Sriprakash and P R Smeesters

Clinical Microbiology and Infection, Vol.19(5), pp.E222-E229

2013

DOI: https://doi.org/10.1111/1469-0691.12134

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Abstract

streptococcus pyogened

m protein

virulence

epidemiology

typing

vaccine

Background: Group A Streptococcus (GAS) M protein is an important virulence factor and potential vaccine antigen, and constitutes the basis for strain typing (emm-typing). Although >200 emm-types are characterized, structural data were obtained from only a limited number of emm-types. We aim to evaluate the sequence diversity of near-full-length M proteins from worldwide sources and analyse their structure, sequence conservation and classification. Methods: GAS isolates recovered from throughout the world during the last two decades underwent emm-typing and complete emm gene sequencing. Predicted amino acid sequence analyses, secondary structure predictions and vaccine epitope mapping were performed using MUSCLE and Geneious software. Results: 1086 isolates from 31 countries were analysed, representing 175 emm-types. Emm-type is predictive of the whole protein structure, independent of geographic origin or clinical association. Findings of an emm-type paired with multiple, highly divergent central regions were not observed. M protein sequence length, the presence or absence of sequence repeats, and predicted secondary structure was assessed in the context of the latest vaccine developments. Conclusions: Based on these global data, the M6 protein model is updated to a three representative M protein (M5, M80, M77) model, to aid in epidemiological analysis, vaccine development and M protein-related pathogenesis studies.

Details

Title: Updated model of group A Streptococcus M proteins based on a comprehensive worldwide study
Authors: David J McMillan (Author) - Queensland Institute of Medical Research
P A Drèze (Author) - Université Libre de Bruxelles, Belgium
T Vu (Author) - Queensland Institute of Medical Research
D E Bessen (Author) - New York Medical College, United States
J Guglielmini (Author) - Institut Pasteur, France
A C Steer (Author) - University of Melbourne
J R Carapetis (Author) - University of Western Australia
L Van Melderen (Author) - Université Libre de Bruxelles, Belgium
K S Sriprakash (Author) - Queensland Institute of Medical Research
P R Smeesters (Author) - Queensland Institute of Medical Research
Publication details: Clinical Microbiology and Infection, Vol.19(5), pp.E222-E229
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2013
DOI: 10.1111/1469-0691.12134
ISSN: 1198-743X
Organisation Unit: University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450294602621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Infectious Diseases; Microbiology

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