Ultrastructure of the Epithelial Cells Associated with Tooth Biomineralization in the Chiton Acanthopleura hirtosa

Jeremy A Shaw; D J Macey; Lesley R Brooker; E J Stockdale; M Saunders; P L Clode

doi:10.1017/S1431927609090230

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Ultrastructure of the Epithelial Cells Associated with Tooth Biomineralization in the Chiton Acanthopleura hirtosa

Journal article

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Peer reviewed

Ultrastructure of the Epithelial Cells Associated with Tooth Biomineralization in the Chiton Acanthopleura hirtosa

Jeremy A Shaw, D J Macey, Lesley R Brooker, E J Stockdale, M Saunders and P L Clode

Microscopy and Microanalysis, Vol.15(2), pp.154-165

2009

DOI: https://doi.org/10.1017/S1431927609090230

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Abstract

TEM

EFTEM

EELS

microwave

iron

ferritin

organic matrix

magnetite

The cusp epithelium is a specialized branch of the superior epithelium that surrounds the developing teeth of chitons and is responsible for delivering the elements required for the formation of biominerals within the major lateral teeth. These biominerals are deposited within specific regions of the tooth in sequence, making it possible to conduct a row by row examination of cell development in the cusp epithelium as the teeth progress from the unmineralized to the mineralized state. Cusp epithelium from the chiton Acanthopleura hirtosa was prepared using conventional chemical and microwave assisted tissue processing, for observation by light microscopy, conventional transmission electron microscopy (TEM) and energy filtered TEM. The onset of iron mineralization within the teeth, initiated at row 13, is associated with a number of dramatic changes in the ultrastructure of the apical cusp cell epithelium. Specifically, the presence of ferritin containing siderosomes, the position and number of mitochondria, and the structure of the cell microvilli are each linked to aspects of the mineralization process. These changes in tissue development are discussed in context with their influence over the physiological conditions within both the cells and extracellular compartment of the tooth at the onset of iron mineralization.

Details

Title: Ultrastructure of the Epithelial Cells Associated with Tooth Biomineralization in the Chiton Acanthopleura hirtosa
Authors: Jeremy A Shaw (Author) - University of Western Australia
D J Macey (Author) - Murdoch University
Lesley R Brooker (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
E J Stockdale (Author) - University of Western Australia
M Saunders (Author) - University of Western Australia
P L Clode (Author) - University of Western Australia
Publication details: Microscopy and Microanalysis, Vol.15(2), pp.154-165
Publisher: Cambridge University Press
Date published: 2009
DOI: 10.1017/S1431927609090230
ISSN: 1431-9276
Organisation Unit: University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99449984402621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Materials Science, Multidisciplinary; Microscopy

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