Type I collagen packing, conformation of the triclinic unit cell

Tim J Wess; A Hammersley; L Wess; A Miller

doi:10.1016/S0022-2836(95)80065-4

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Type I collagen packing, conformation of the triclinic unit cell

Journal article

Peer reviewed

Type I collagen packing, conformation of the triclinic unit cell

Tim J Wess, A Hammersley, L Wess and A Miller

Journal of Molecular Biology, Vol.248(2), pp.487-493

1995

DOI: https://doi.org/10.1016/S0022-2836(95)80065-4

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Abstract

type I collagen

X-ray diffraction

triclinic

structure

synchrotron

The X-ray diffraction pattern of tendon collagen can contain a number ofsharp Bragg peaks indicating three-dimensional crystallinity of the sample. Optimal diffraction images have been obtained with a high flux synchrotron X-ray source and a carefully maintained sample environment and staining techniques. The Bragg peaks are always superimposed on a diffuse background. This makes interpretation of data difficult and a number of conflicting models of collagen packing have been proposed. The removal of the diffuse scatter from the images allows the Bragg peaks to be seen on a relatively flat background. This was conducted by modelling the background points as a series of two-dimensional polynomial functions. The resultant set of observed Bragg reflections serves as an excellent basis to test the validity of two contradictory packing modes; (1) the triclinic model, Fraser et al., (2) the microfibril model, Kajava. From this it can easily be seen that the model proposed by Kajava is inappropriate, since there is limited agreement between predicted positions of reflections and the positions of observable reflections on film. The packing of collagen molecules on a triclinic lattice is favoured by this criterion. © 1995 Academic Press Limited.

Details

Title: Type I collagen packing, conformation of the triclinic unit cell
Authors: Tim J Wess (Author) - University of Stirling, United Kingdom
A Hammersley (Author) - ESRF, France
L Wess (Author) - University of Stirling, United Kingdom
A Miller (Author) - University of Stirling, United Kingdom
Publication details: Journal of Molecular Biology, Vol.248(2), pp.487-493
Publisher: Academic Press
Date published: 1995
DOI: 10.1016/S0022-2836(95)80065-4
ISSN: 0022-2836
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99450648202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Biochemistry & Molecular Biology