Two Novel Mosquitocidal Peptides Isolated from the Venom of the Bahia Scarlet Tarantula (Lasiodora klugi)

Jamila  Ahmed; Andrew A Walker; Hugo D.  Perdomo; Shaodong Guo; Samantha A Nixon; Irina Vetter; Hilary I.  Okoh; Dalhatu M.  Shehu; Mohammed N.  Shuaibu; Iliya S.  Ndams; Glenn F King; Volker Herzig

doi:10.3390/toxins15070418

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Two Novel Mosquitocidal Peptides Isolated from the Venom of the Bahia Scarlet Tarantula (Lasiodora klugi)

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Two Novel Mosquitocidal Peptides Isolated from the Venom of the Bahia Scarlet Tarantula (Lasiodora klugi)

Jamila Ahmed, Andrew A Walker, Hugo D. Perdomo, Shaodong Guo, Samantha A Nixon, Irina Vetter, Hilary I. Okoh, Dalhatu M. Shehu, Mohammed N. Shuaibu, Iliya S. Ndams, …

Toxins, Vol.15(7), pp.1-14

2023

DOI: https://doi.org/10.3390/toxins15070418

PMCID: PMC10467143

PMID: 37505687

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Abstract

Lasiodora klugi

insecticidal toxin

Aedes aegypti

disulfide-directed β-hairpin

inhibitor cystine knot

Effective control of diseases transmitted by Aedes aegypti is primarily achieved through vector control by chemical insecticides. However, the emergence of insecticide resistance in A. aegypti undermines current control efforts. Arachnid venoms are rich in toxins with activity against dipteran insects and we therefore employed a panel of 41 spider and 9 scorpion venoms to screen for mosquitocidal toxins. Using an assay-guided fractionation approach, we isolated two peptides from the venom of the tarantula Lasiodora klugi with activity against adult A. aegypti. The isolated peptides were named U-TRTX-Lk1a and U-TRTX-Lk2a and comprised 41 and 49 residues with monoisotopic masses of 4687.02 Da and 5718.88 Da, respectively. U-TRTX-Lk1a exhibited an LD50 of 38.3 pmol/g when injected into A. aegypti and its modeled structure conformed to the inhibitor cystine knot motif. U-TRTX-Lk2a has an LD50 of 45.4 pmol/g against adult A. aegypti and its predicted structure conforms to the disulfide-directed β-hairpin motif. These spider-venom peptides represent potential leads for the development of novel control agents for A. aegypti.

Details

Title: Two Novel Mosquitocidal Peptides Isolated from the Venom of the Bahia Scarlet Tarantula (Lasiodora klugi)
Authors: Jamila Ahmed (Author) - Ahmadu Bello University
Andrew A Walker (Author) - The University of Queensland
Hugo D. Perdomo (Author) - The University of Queensland
Shaodong Guo (Author) - The University of Queensland
Samantha A Nixon (Author) - The University of Queensland
Irina Vetter (Author) - The University of Queensland
Hilary I. Okoh (Author) - Federal University Oye Ekiti
Dalhatu M. Shehu (Author) - Ahmadu Bello University
Mohammed N. Shuaibu (Author) - Ahmadu Bello University
Iliya S. Ndams (Author) - Ahmadu Bello University
Glenn F King (Author) - The University of Queensland
Volker Herzig (Corresponding Author) - University of the Sunshine Coast, Queensland, School of Science, Technology and Engineering
Publication details: Toxins, Vol.15(7), pp.1-14
Publisher: MDPI AG
Date published: 2023
DOI: 10.3390/toxins15070418
ISSN: 2072-6651
PMID: 37505687; PMC10467143
Copyright note: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Grants: Be(e) friendly venomous spiders! Novel biopesticides from arachnid venoms, FT190100482, Australian Research Council (Australia, Canberra) - ARC
ARC Centre of Excellence for Innovations in Peptide and Protein Science, CE200100012, Australian Research Council (Australia, Canberra) - ARC
Venoms to drugs: translating venom peptides into human therapeutics, 1136889, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Mechanistic and pharmacological insights into peripheral sensory neuron function in physiological and pathological pain, 2017086, National Health and Medical Research Council (Australia, Canberra) - NHMRC
Nettles & toxic toupees: the molecular weaponry of venomous caterpillars, DP200102867, Australian Research Council (Australia, Canberra) - ARC
Grant note: International Foundation for Science Sweden (I-1-F-6130-1) and the Institute for Molecular Bioscience, The University of Queensland, Australia.
Organisation Unit: School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99738098802621
Output Type: Journal article

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