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Trichinella spiralis secretes abundant unencapsulated small RNAs with potential effects on host gene expression2.14 MB
Published VersionCC BY V4.0, Open Access
Journal article
Trichinella spiralis secretes abundant unencapsulated small RNAs with potential effects on host gene expression
International Journal for Parasitology, Vol.50(9), pp.697-705
2020
PMID: 32622688
Abstract
Many organisms, including parasitic nematodes, secrete small RNAs into the extracellular environment, largely encapsulated within small vesicles. Parasite-secreted material often contains microRNAs (miRNAs), raising the possibility that they might regulate host genes in target cells. Here we characterise secreted RNAs from the parasitic nematode Trichinella spiralis at two different life stages. We show that adult T. spiralis, which inhabit intestinal mucosa, secrete miRNAs within vesicles. Unexpectedly, T. spiralis muscle stage larvae, which live intracellularly within skeletal muscle cells, secrete miRNAs that appear not to be encapsulated. Notably, secreted miRNAs include a homologue of mammalian miRNA-31, which has an important role in muscle development. Our work therefore suggests that RNAs may be secreted without encapsulation in vesicles, with implications for the biology of T. spiralis infection.
Details
- Title
- Trichinella spiralis secretes abundant unencapsulated small RNAs with potential effects on host gene expression
- Authors
- Peter J Taylor (Author) - Imperial College LondonJana Hagen (Author) - Imperial College LondonFarid N Faruqu (Author) - King's College LondonKhuloud T Al-Jamal (Author) - King's College LondonBonnie Quigley (Author) - Imperial College LondonMorgan Beeby (Author) - Imperial College LondonMurray E Selkirk (Author) - Imperial College LondonPeter Sarkies (Author) - Imperial College London
- Publication details
- International Journal for Parasitology, Vol.50(9), pp.697-705
- Publisher
- Elsevier Ltd
- Date published
- 2020
- DOI
- 10.1016/j.ijpara.2020.05.008
- ISSN
- 1879-0135; 0020-7519
- PMID
- 32622688
- Copyright note
- © 2020 The Authors. Published by Elsevier Ltd on behalf of Australian Society for Parasitology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
- Organisation Unit
- University of the Sunshine Coast, Queensland; Thompson Institute; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99483608102621
- Output Type
- Journal article
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- Parasitology
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