Transcriptomic changes during previtellogenic and vitellogenic stages of ovarian development in wreckfish (hāpuku), Polyprion oxygeneios (Perciforms)

Matthew J Wylie; Jane E Symonds; Alvin N Setiawan; Glen W Irvine; Hui Liu; Abigail Elizur; P Mark Lokman

doi:10.3390/fishes4010016

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Transcriptomic changes during previtellogenic and vitellogenic stages of ovarian development in wreckfish (hāpuku), Polyprion oxygeneios (Perciforms)

Journal article

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Transcriptomic changes during previtellogenic and vitellogenic stages of ovarian development in wreckfish (hāpuku), Polyprion oxygeneios (Perciforms)

Matthew J Wylie, Jane E Symonds, Alvin N Setiawan, Glen W Irvine, Hui Liu, Abigail Elizur and P Mark Lokman

Fishes, Vol.4(1), 16

2019

DOI: https://doi.org/10.3390/fishes4010016

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Abstract

Polyprion

transcriptome

vitellogenesis

RNA-Seq

oogenesis

hapuku

de novo

steroid

lipid

‘Wreckfish’ a collective of species belonging to the family Polyprionidae, are an important commercial fishery and have significant aquaculture potential. Until now, genomic or transcriptomic information for any species within the genus Polyprion has either remained unpublished or is non-existent. Using Illumina HiSeq, we compared the transcriptomes of h¯apuku (Polyprion oxygeneios) ovaries to explore developmental stage-specific variations underlying their reproductive physiology. We sought to identify differentially expressed genes and the associated shifts in biological pathways between previtellogenic and early vitellogenic ovaries. Ovarian tissue was repeatedly biopsied by gonopore cannulation from the same females (n = 3) throughout oogenesis. Reproductive status of initial biopsies was confirmed as being previtellogenic and that in biopsies collected eight weeks later as early vitellogenic. A de novo h¯apuku transcriptome was assembled (146,189 transcripts) from RNA-Seq data without a reference genome. On average, each tissue sample contained 17.5 million trimmed reads. Gene annotation was 80% when using BLASTX against Genbank Non Redundant database. Fifty-three transcripts were differentially expressed within the FDR of 0.05 when previtellogenic and early vitellogenic ovaries were compared; this reduced to 35 differentially expressed genes when transcript duplications were pooled. Among these were genes tentatively associated with the electron transport chain, lipid metabolism, steroidogenesis and mineral/solute transportation. These data provide a snap-shot into stage-specific physiological events during ogenesis in the ovary of a teleost and an extensive molecular resource for research on species in the Genus Polyprion.

Details

Title: Transcriptomic changes during previtellogenic and vitellogenic stages of ovarian development in wreckfish (hāpuku), Polyprion oxygeneios (Perciforms)
Authors: Matthew J Wylie (Author) - University of Otago, New Zealand
Jane E Symonds (Author) - Northland Marine Research Centre, New Zealand
Alvin N Setiawan (Author) - Northland Marine Research Centre, New Zealand
Glen W Irvine (Author) - Northland Marine Research Centre, New Zealand
Hui Liu (Author) - University of Otago, New Zealand
Abigail Elizur (Author) - University of the Sunshine Coast
P Mark Lokman (Author) - University of Otago, New Zealand
Publication details: Fishes, Vol.4(1), 16; 15
Publisher: MDPI AG
Date published: 2019
DOI: 10.3390/fishes4010016
ISSN: 2410-3888
Copyright note: Copyright © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99450634202621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Fisheries; Marine & Freshwater Biology

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