Transcriptomic and nutritional analysis of the hepatopancreas in cultured juvenile Panulirus ornatus throughout the moult cycle

Courtney L. Lewis; Basseer M. Codabaccus; Chris G. Carter; Quinn P. Fitzgibbon; Gregory G. Smith; Abigail Elizur; Tomer Ventura

doi:10.1016/j.aqrep.2024.102477

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Transcriptomic and nutritional analysis of the hepatopancreas in cultured juvenile Panulirus ornatus throughout the moult cycle

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Transcriptomic and nutritional analysis of the hepatopancreas in cultured juvenile Panulirus ornatus throughout the moult cycle

Courtney L. Lewis, Basseer M. Codabaccus, Chris G. Carter, Quinn P. Fitzgibbon, Gregory G. Smith, Abigail Elizur and Tomer Ventura

Aquaculture Reports, Vol.39, pp.1-11

2024

DOI: https://doi.org/10.1016/j.aqrep.2024.102477

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Abstract

moult cycle

nutritional condition

hepatopancreas

transcriptomics

lipid metabolism

Panulirus ornatus

Juveniles of the ornate spiny lobster, Panulirus ornatus, grow rapidly through frequent moulting events, requiring them to accumulate sufficient nutrient reserves to fuel the energetically demanding process of ecdysis. As moulting events occur so often, especially in the early-stage juveniles, it is critical that the juveniles maintain adequate nutritional condition throughout the moult cycle for maximum survival. This is especially important for animals housed together on a larger scale within commercial culture environments for ensuring maximum survival. In this study, the nutritional condition of juvenile P. ornatus examined over the duration of a moult cycle from the 7th to 8th juvenile moulting events. Nutritional condition was measured through biochemical composition analysis as well as histological and transcriptomic examination of the hepatopancreas. The rapid accumulation of nutrient reserves was evident by the increase of dry matter and gross energy over the moult cycle. Six days following ecdysis, there was a significant reduction in total lipids consistent with a downregulation of genes associated with lipid metabolism. Histological examination of the hepatopancreas did not illustrate any changes in the cell distribution or morphology associated with the consistent drop in total lipids and associated metabolic pathways suggesting the involvement of other regulatory mechanisms. The present study is the first to investigate biochemical changes over the duration of a moult cycle in early juvenile stages of P. ornatus, imperative for enhancing the understanding of energy demands of undergoing frequent ecdysis and provides insights into nutrient utilisation for this species.

Details

Title: Transcriptomic and nutritional analysis of the hepatopancreas in cultured juvenile Panulirus ornatus throughout the moult cycle
Authors: Courtney L. Lewis - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Basseer M. Codabaccus - University of Tasmania
Chris G. Carter - University of Tasmania
Quinn P. Fitzgibbon - University of Tasmania
Gregory G. Smith - University of Tasmania
Abigail Elizur - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Tomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
Publication details: Aquaculture Reports, Vol.39, pp.1-11
Publisher: Elsevier BV
Date published: 2024
DOI: 10.1016/j.aqrep.2024.102477
ISSN: 2352-5134
Data Availability: Data deposited in public databases.
Grants: The ARC Research Hub for Sustainable Lobster Aquaculture, IH190100014, Australian Research Council (Australia, Canberra) - ARC
Organisation Unit: School of Education and Tertiary Access; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 991076422902621
Output Type: Journal article

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