Transcriptomic analysis of the autophagy machinery in crustaceans

Saowaros Suwansa-ard; Wilairat Kankuan; Tipsuda Thongbuakaew; Jirawat Saetan; Napamanee Kornthong; Thanapong Kruangkum; Kanjana Khornchatri; Scott F Cummins; Ciro Isidoro; Prasert Sobhon

doi:10.1186/s12864-016-2996-4

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Transcriptomic analysis of the autophagy machinery in crustaceans

Journal article

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Transcriptomic analysis of the autophagy machinery in crustaceans

Saowaros Suwansa-ard, Wilairat Kankuan, Tipsuda Thongbuakaew, Jirawat Saetan, Napamanee Kornthong, Thanapong Kruangkum, Kanjana Khornchatri, Scott F Cummins, Ciro Isidoro and Prasert Sobhon

BMC Genomics, Vol.17, 587

2016

DOI: https://doi.org/10.1186/s12864-016-2996-4

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Abstract

autophagy

crustaceans

transcriptome

nervous system

ovary

autophagy markers

Background: The giant freshwater prawn, Macrobrachium rosenbergii, is a decapod crustacean that is commercially important as a food source. Farming of commercial crustaceans requires an efficient management strategy because the animals are easily subjected to stress and diseases during the culture. Autophagy, a stress response process, is well-documented and conserved in most animals, yet it is poorly studied in crustaceans. Results: In this study, we have performed an in silico search for transcripts encoding autophagy-related (Atg) proteins within various tissue transcriptomes of M. rosenbergii. Basic Local Alignment Search Tool (BLAST) search using previously known Atg proteins as queries revealed 41 transcripts encoding homologous M. rosenbergii Atg proteins. Among these Atg proteins, we selected commonly used autophagy markers, including Beclin 1, vacuolar protein sorting (Vps) 34, microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B), p62/sequestosome 1 (SQSTM1), and lysosomal-associated membrane protein 1 (Lamp-1) for further sequence analyses using comparative alignment and protein structural prediction. We found that crustacean autophagy marker proteins contain conserved motifs typical of other animal Atg proteins. Western blotting using commercial antibodies raised against human Atg marker proteins indicated their presence in various M. rosenbergii tissues, while immunohistochemistry localized Atg marker proteins within ovarian tissue, specifically late stage oocytes. Conclusions: This study demonstrates that the molecular components of autophagic process are conserved in crustaceans, which is comparable to autophagic process in mammals. Furthermore, it provides a foundation for further studies of autophagy in crustaceans that may lead to more understanding of the reproduction- and stress-related autophagy, which will enable the efficient aquaculture practices.

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Title: Transcriptomic analysis of the autophagy machinery in crustaceans
Authors: Saowaros Suwansa-ard (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Wilairat Kankuan (Author) - Madihol University, Thailand
Tipsuda Thongbuakaew (Author) - Madihol University, Thailand
Jirawat Saetan (Author) - Prince of Songkla, Thailand
Napamanee Kornthong (Author) - Thammasat University, Thailand
Thanapong Kruangkum (Author) - Madihol University, Thailand
Kanjana Khornchatri (Author) - Madihol University, Thailand
Scott F Cummins (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
Ciro Isidoro (Author) - Amedeo Avogadro University, Thailand
Prasert Sobhon (Author) - Madihol University, Thailand
Publication details: BMC Genomics, Vol.17, 587; 14
Publisher: BioMed Central Ltd.
Date published: 2016
DOI: 10.1186/s12864-016-2996-4
ISSN: 1471-2164
Copyright note: Copyright © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450348702621
Output Type: Journal article

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Web Of Science research areas: Biotechnology & Applied Microbiology; Genetics & Heredity

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