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Journal article
Transcriptomic Analysis across Crayfish (Cherax quadricarinatus) Claw Regeneration Reveals Potential Stem Cell Sources for Cultivated Crustacean Meat
International Journal of Molecular Sciences, Vol.25(16), pp.1-31
2024
Abstract
In the face of rising global demand and unsustainable production methods, cultivated crustacean meat (CCM) is proposed as an alternative means to produce delicious lobster, shrimp, and crab products. Cultivated meat requires starting stem cells that may vary in terms of potency and the propensity to proliferate or differentiate into myogenic (muscle-related) tissues. Recognizing that regenerating limbs are a non-lethal source of tissue and may harbor relevant stem cells, we selected those of the crayfish Cherax quadricarinatus as our model. To investigate stem cell activity, we conducted RNA-Seq analysis across six stages of claw regeneration (four pre-molt and two post-molt stages), along with histology and real-time quantitative PCR (qPCR). Our results showed that while genes related to energy production, muscle hypertrophy, and exoskeletal cuticle synthesis dominated the post-molt stages, growth factor receptors (FGFR, EGFR, TGFR, and BMPR) and those related to stem cell proliferation and potency (Cyclins, CDKs, Wnts, C-Myc, Klf4, Sox2, PCNA, and p53) were upregulated before the molt. Pre-molt upregulation in several genes occurred in two growth peaks; Stages 2 and 4. We therefore propose that pre-molt limb regeneration tissues, particularly those in the larger Stage 4, present a prolific and non-lethal source of stem cells for CCM development.
Details
- Title
- Transcriptomic Analysis across Crayfish (Cherax quadricarinatus) Claw Regeneration Reveals Potential Stem Cell Sources for Cultivated Crustacean Meat
- Authors
- Lisa Musgrove - University of the Sunshine Coast, Queensland, Centre for BioinnovationAvani Bhojwani - University of the Sunshine Coast, Queensland, Centre for BioinnovationCameron Hyde - The University of QueenslandSusan Glendinning - University of the Sunshine Coast, Queensland, Centre for BioinnovationJosephine Nocillado - University of the Sunshine Coast, Queensland, Centre for BioinnovationFraser D. Russell - University of the Sunshine Coast, Queensland, Centre for BioinnovationTomer Ventura (Corresponding Author) - University of the Sunshine Coast, Queensland, Centre for Bioinnovation
- Publication details
- International Journal of Molecular Sciences, Vol.25(16), pp.1-31
- Publisher
- MDPI AG
- Date published
- 2024
- DOI
- 10.3390/ijms25168623
- ISSN
- 1422-0067
- Copyright note
- © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- Data Availability
- The FASTQ files are available through NCBI Sequence Read Archive under project number PRJNA780617 and relative expression data are available through CrustyBase and in Supplementary Data File SF3.
- Grant note
- The authors declare that this study received funding from Shiok Meats and New Harvest.
- Organisation Unit
- School of Health - Biomedicine; Healthy Ageing Research Cluster; School of Science, Technology and Engineering; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 991054379802621
- Output Type
- Journal article
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