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Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis4.23 MB
Published VersionCC BY V4.0, Open Access
Journal article
Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
Frontiers in Immunology, Vol.12, pp.1-19
2021
PMID: 34925353
Abstract
Scabies, a human skin infestation caused by the ectoparasitic mite Sarcoptes scabiei var. hominis, affects more than 200 million people globally. The prevailing knowledge of the disease process and host immune response mechanisms is limited. A better understanding of the host-parasite relationship is essential for the identification of novel vaccine and drug targets. Here we aimed to interrogate the transcriptomic profiles of mite-infested human skin biopsies with clinical manifestations of ordinary scabies subjects (“OS”; n = 05) and subjects naive to scabies (“control”; n = 03) using RNASeq data analysis. A combined clustering, network, and pathway mapping approach enabled us to identify key signaling events in the host immune and pro-inflammatory responses to S. scabiei infestation. The clustering patterns showed various differentially expressed genes including inflammatory responses and innate immunity genes (DEFB4A, IL-19, CXCL8, CSF3, SERPINB4, S100A7A, HRNR) and notably upregulation of the JAK-STAT pathway in scabies-infested samples. Mite-infested human skin biopsies (GSE178563) were compared with an ex-vivo porcine infested model (E-MTAB-6433) and human skin equivalents (GSE48459). Marked enrichment of immune response pathways (JAK-STAT signaling, IL-4 and IL-13 pathway, and Toll receptor cascade), chemokine ligands and receptors (CCL17, CCL18, CCL3L1, CCL3L3, CCR7), and cytokines (IL-13 and IL-20) were observed. Additionally, genes known for their role in psoriasis and atopic dermatitis were upregulated, e.g., IL-19. The detailed transcriptomic profile has provided an insight into molecular functions, biological processes, and immunological responses and increased our understanding about transcriptomic regulation of scabies in human.
Details
- Title
- Transcriptome Analysis of Host Inflammatory Responses to the Ectoparasitic Mite Sarcoptes scabiei var. hominis
- Authors
- Huma Shehwana (Author) - National University of Medical SciencesSadaf Ijaz (Author) - National University of Sciences and TechnologyAbeera Fatima (Author) - National University of Sciences and TechnologyShelley Walton (Author) - University of the Sunshine Coast, Queensland, School of Health and Sport Sciences - LegacyZafar Iqbal Sheikh (Author) - Military HospitalWaseem Haider (Author) - COMSATS University IslamabadShumaila Naz (Author) - National University of Medical Sciences
- Publication details
- Frontiers in Immunology, Vol.12, pp.1-19
- Publisher
- Frontiers Research Foundation
- Date published
- 2021
- DOI
- 10.3389/fimmu.2021.778840
- ISSN
- 1664-3224
- PMID
- 34925353
- Copyright note
- Copyright © 2021 Shehwana, Ijaz, Fatima, Walton, Sheikh, Haider and Naz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Organisation Unit
- University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy
- Language
- English
- Record Identifier
- 99597008702621
- Output Type
- Journal article
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