Transcriptional regulation of a pineapple polyphenol oxidase gene and its relationship to blackheart

Yuchan Zhou; Tim J O'Hare; Marcelle Jobin-Décor; Steven J R Underhill; Ron B H Wills; Michael W Graham

doi:10.1046/j.1467-7652.2003.00042.x

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Transcriptional regulation of a pineapple polyphenol oxidase gene and its relationship to blackheart

Journal article

Open access

Peer reviewed

Transcriptional regulation of a pineapple polyphenol oxidase gene and its relationship to blackheart

Yuchan Zhou, Tim J O'Hare, Marcelle Jobin-Décor, Steven J R Underhill, Ron B H Wills and Michael W Graham

Plant Biotechnology Journal, Vol.1(6), pp.463-478

2003

DOI: https://doi.org/10.1046/j.1467-7652.2003.00042.x

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Abstract

Technology

Biological Sciences

Medical and Health Sciences

blackheart

gene regulation

gibberellin

polyphenol oxidase

pineapple

promoter

Two genes encoding polyphenol oxidase (PPO) were isolated from pineapple (Ananas comosus[L.] Merr. cv. Smooth Cayenne). Sequence analyses showed that both contained a single intron and encoded typical chloroplast-localized PPO proteins, the sequences of which corresponded to two pineapple PPO cDNAs, PINPPO1 and PINPPO2, recently described by Stewart et al. (2001). Southern blot analyses suggested that pineapple contained only two PPO genes. Analysis of expression of PINPPO1 promoter GUS fusion constructs showed this promoter had a low basal activity and was cold- and wound-inducible, consistent with known mRNA expression profiles. Striking homologies to gibberellin response complexes (GARC) were observed in sequences of both the PINPPO1 and PINPPO2 promoters. Transient assays in mature pineapple fruit and stable expression in transgenic tobacco showed that PINPPO1 promoter-GUS fusions were indeed gibberellin (GA) responsive. A role for the element within the putative GARCs in mediating GA-responsiveness of the PINPPO1 promoter was confirmed by mutational analysis. PINPPO2 was also shown to be GA-responsive by RT-PCR analysis. Mutant PINPPO1 promoter-GUS fusion constructs, which were no longer GA-inducible, showed a delayed response to cold induction in pineapple fruit in transient assays, suggesting a role for GA in blackheart development. This was supported by observations that exogenous GA3 treatment induced blackheart in the absence of chilling. Sequences showing homology to GARCs are also present in some PPO promoters in tomato, suggesting that GA regulates PPO expression in diverse species.

Details

Title: Transcriptional regulation of a pineapple polyphenol oxidase gene and its relationship to blackheart
Authors: Yuchan Zhou (Author) - University of Queensland
Tim J O'Hare (Author) - Department of Primary Industries
Marcelle Jobin-Décor (Author) - University of Queensland
Steven J R Underhill (Author) - Department of Primary Industries
Ron B H Wills (Author) - University of Newcastle
Michael W Graham (Author) - University of Queensland
Publication details: Plant Biotechnology Journal, Vol.1(6), pp.463-478
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2003
DOI: 10.1046/j.1467-7652.2003.00042.x
ISSN: 1467-7644
Copyright note: Copyright © 2003 Blackwell Publishing Ltd. This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Organisation Unit: Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; Sustainability Research Cluster
Language: English
Record Identifier: 99449720502621
Output Type: Journal article

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Collaboration types: Domestic collaboration
Web Of Science research areas: Biotechnology & Applied Microbiology; Plant Sciences