Transcriptional profiling of spiny lobster metamorphosis reveals three new additions to the nuclear receptor superfamily

Cameron J Hyde; Quinn P Fitzgibbon; Abigail Elizur; Gregory G Smith; Tomer Ventura

doi:10.1186/s12864-019-5925-5

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Transcriptional profiling of spiny lobster metamorphosis reveals three new additions to the nuclear receptor superfamily

Journal article

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Transcriptional profiling of spiny lobster metamorphosis reveals three new additions to the nuclear receptor superfamily

Cameron J Hyde, Quinn P Fitzgibbon, Abigail Elizur, Gregory G Smith and Tomer Ventura

BMC Genomics, Vol.20, pp.1-14

2019

DOI: https://doi.org/10.1186/s12864-019-5925-5

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Abstract

Background: The Crustacea are an evolutionarily diverse taxon which underpins marine food webs and contributes significantly to the global economy. However, our knowledge of crustacean endocrinology and development is far behind that of terrestrial arthropods. Here we present a unique insight into the molecular pathways coordinating crustacean metamorphosis, by reconciling nuclear receptor (NR) gene activity from a 12-stage, 3-replicate transcriptome in the ornate spiny lobster (Panulirus ornatus) during larval development. Results: We annotated 18 distinct nuclear receptor genes, including three novel NRs which are upregulated prior to metamorphosis and have hence been named the "molt-associated receptors" (MARs). We also demonstrate the ecdysone-responsive expression of several known molt-related NRs including ecdysone receptor, fushi-tarazu-F1 and E75. Phylogenetic analysis of the curated NR family confirmed gene annotations and suggested that the MARs are a recent addition to the crustacean superfamily, occurring across the Malacostraca from the Stomatopoda to the Decapoda. The ligand-binding domain of these receptors appears to be less conserved than that of typical group-1 NRs. Expression data from two other crustacean species was utilized to examine MAR expression. The Y-organ of the tropical land crab showed a decline in expression of all MARs from intermolt to post-molt. Tissue distributions showed gonad-enriched expression in the Eastern rock lobster and antennal gland-enriched expression in the tropical land crab, although expression was evident across most tissues. Conclusion: By mining transcriptome data, we have curated an extensive list of NR genes expressed during the metamorphic molts of P. ornatus, including three novel crustacean NRs which appear to play a role in the molting process. Divergence of the E-region of these new receptors indicates that they may have adopted a function that is unconventional for NRs. Based on expression patterns, we can confirm that a number of NRs play a role in the ecdysone cassette which regulates molting in crustaceans. This study describes in detail the molecular events surrounding crustacean molting and metamorphosis by taking advantage of the distinctive life history unique to achelatan crustaceans.

Details

Title: Transcriptional profiling of spiny lobster metamorphosis reveals three new additions to the nuclear receptor superfamily
Authors: Cameron J Hyde (Author) - University of the Sunshine Coast
Quinn P Fitzgibbon (Author) - University of Tasmania
Abigail Elizur (Author) - University of the Sunshine Coast
Gregory G Smith (Author) - University of Tasmania
Tomer Ventura (Author) - University of the Sunshine Coast
Publication details: BMC Genomics, Vol.20, pp.1-14
Publisher: BioMed Central Ltd.
Date published: 2019
DOI: 10.1186/s12864-019-5925-5
ISSN: 1471-2164
Copyright note: Copyright © The Author(s). 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, andreproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Organisation Unit: School of Science and Engineering - Legacy; School of Education and Tertiary Access; University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99451005702621
Output Type: Journal article

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