Journal article
Topophysic effects differ between node and organogenic cultures of the eucalypt Corymbia torelliana × C. citriodora
Plant Cell, Tissue and Organ Culture, Vol.104(1), pp.69-77
2011
Abstract
The eucalypt Corymbia torelliana × C. citriodora is planted widely in India, Brazil and Australia although plantation establishment has been limited by inadequate seed supply and low amenability to propagation via cuttings. This study optimised node culture and organogenic culture methods for in vitro propagation of Corymbia hybrids by identifying explant position (topophysic) effects on rooting, shoot elongation and shoot proliferation. Strong, negative morphogenic gradients in shoot elongation and proliferation capacity were evident from the cotyledonary node to the fourth or fifth node of seedlings when their nodes were transferred to node culture (without benzyladenine). These topophysic effects were related to differences in rooting capacity of individual nodes. Root formation in node culture was associated with formation of long multi-nodal axillary shoots, and so higher rooting of shoots from the cotyledonary node or first true-leaf node was associated with higher shoot proliferation. However, all nodes were equally capable of shoot proliferation in organogenic culture (with 2.2 μM benzyladenine), where rooting and rapid stem elongation did not occur. Most shoots (61-100%) from both node culture and organogenic culture were converted to plantlets, with plantlet conversion and primary root number not differing significantly among explant node positions. The strong topophysic effect in node culture, combined with the lack of a topophysic effect in organogenic culture, provides for an optimised clonal propagation system based on segregation of nodes from the same seedling into separate node and organogenic culture pathways.
Details
- Title
- Topophysic effects differ between node and organogenic cultures of the eucalypt Corymbia torelliana × C. citriodora
- Authors
- Cao Dinh Hung (Author) - University of the Sunshine Coast - Faculty of Science, Health and EducationStephen J Trueman (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
- Publication details
- Plant Cell, Tissue and Organ Culture, Vol.104(1), pp.69-77
- Publisher
- Springer Netherlands
- Date published
- 2011
- DOI
- 10.1007/s11240-010-9805-6
- ISSN
- 0167-6857
- Copyright note
- Copyright © 2012 Springer Netherlands. The Author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final definitive publication is available at www.springerlink.com
- Organisation Unit
- School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
- Language
- English
- Record Identifier
- 99449526202621
- Output Type
- Journal article
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