Journal article
Tonoplast lipid composition and proton pump of pineapple fruit during low temperature storage and blackheart development
Journal of Membrane Biology, Vol.247(5), pp.429-439
2014
Abstract
Vacuole represents a major storage organelle playing vital roles in pH homeostasis and cellular detoxification. The chemical and functional properties of tonoplast in response to chilling temperature and their roles in chilling injury are largely unknown. In the current study, lipid composition of tonoplast and the activities of two vacuolar proton pumps, H+-ATPase (V-ATPase) and H+-pyrophosphatase (V-PPase) were investigated in accordance with the development of blackheart, a form of chilling injury in pineapple fruit (Ananus comosus). Chilling temperature at 10 oC for 1 week induced irreversible blackheart injury in concurrence with a substantial decrease in V-ATPase activity. By contrast, the activity was increased after 1 week at 25 oC. The activity of V-PPase was not changed under both temperatures. Level of total phospholipids of tonoplast decreased at 10 oC, but increased at 25 oC. There was no change at the level of total glycolipids under both temperatures. Thus low temperature increased the ratio of total glycolipids vs. total phospholipids of tonoplast. Phosphatidylcholine and phosphatidylethanolamine were the predominant phospholipid species of tonoplast. Low temperature increased the relative level of phosphatidic acid but decreased the percentage of both phosphatidylcholine and phosphatidylethanolamine. Unsaturated fatty acids accounted for over 75 % of the total fatty acids of tonoplast, with C18:2 being predominant. Low temperature significantly decreased the percentage of C18:3. Modification of membrane lipid composition and its effect on the functional property of tonoplast at low temperature were discussed in correlation with their roles in the development of chilling injury in pineapple fruit.
Details
- Title
- Tonoplast lipid composition and proton pump of pineapple fruit during low temperature storage and blackheart development
- Authors
- Yuchan Zhou (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and EngineeringX Pan (Author) - Chinese Academy of Sciences, ChinaH Qu (Author) - Chinese Academy of Sciences, ChinaSteven J R Underhill (Author) - University of the Sunshine Coast - Faculty of Science, Health, Education and Engineering
- Publication details
- Journal of Membrane Biology, Vol.247(5), pp.429-439
- Publisher
- Springer New York LLC
- Date published
- 2014
- DOI
- 10.1007/s00232-014-9650-3
- ISSN
- 0022-2631
- Copyright note
- Copyright © 2014 Springer New York LLC. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The final publication is available at Springer via http://dx.doi.org/10.1007/s00232-014-9650-3
- Organisation Unit
- Australian Centre for Pacific Islands Research; University of the Sunshine Coast, Queensland; Sustainability Research Cluster
- Language
- English
- Record Identifier
- 99448620702621
- Output Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Cell Biology
- Physiology