Journal article
Tissue MicroArray (TMA) analysis of normal and persistent Chlamydophila pneumoniae infection
BMC Infectious Diseases, Vol.6, 152
2006
Abstract
Background: Chlamydophila pneumoniae infection has been implicated as a potential risk factor for atherosclerosis, however the mechanism leading to persistent infection and its role in the disease process remains to be elucidated. Methods: We validated the use of tissue microarray (TMA) technology, in combination with immunohistochemistry (IHC), to test antibodies (GroEL, GroES, GspD, Ndk and Pyk) raised against differentially expressed proteins under an interferon-gamma (IFN-γ) induced model of chlamydial persistence. Results: In the cell pellet array, we were able to identify differences in protein expression patterns between untreated and IFN-γ treated samples. Typical, large chlamydial inclusions could be observed in the untreated samples with all antibodies, whereas the number of inclusions were decreased and were smaller and atypical in shape in the IFN-γ treated samples. The staining results obtained with the TMA method were generally similar to the changes observed between normal and IFN-γ persistence using proteomic analysis. Subsequently, it was shown in a second TMA including archival atheromatous heart tissues from 12 patients undergoing heart transplantation, that GroEL, GroES, GspD and Pyk were expressed in atheromatous heart tissue specimens as well, and were detectable morphologically within lesions by IHC. Conclusion: TMA technology proved useful in documenting functional proteomics data with the morphologic distribution of GroEL, GroES, GspD, Ndk and Pyk within formalin-fixed, paraffinembedded cell pellets and tissues from patients with severe coronary atherosclerosis. The antibodies GroEL and GroES, which were upregulated under persistence in proteomic analysis, displayed positive reaction in atheromatous heart tissue from 10 out of 12 patients. These may be useful markers for the detection of persistent infection in vitro and in vivo.
Details
- Title
- Tissue MicroArray (TMA) analysis of normal and persistent Chlamydophila pneumoniae infection
- Authors
- Nicole Borel (Author) - University of Zurich, SwitzerlandSanghamitra Mukhopadhyay (Author) - University of Louisville, United StatesCarmen Kaiser (Author) - University of Zurich, SwitzerlandErin D Sullivan (Author) - University of Louisville, United StatesRichard D Miller (Author) - University of Louisville, United StatesPeter Timms (Author) - Queensland University of TechnologyJames T Summersgill (Author) - University of Louisville, United StatesJulio A Ramirez (Author) - University of Louisville, United StatesAndreas Pospochil (Author) - University of Zurich, Switzerland
- Publication details
- BMC Infectious Diseases, Vol.6, 152
- Publisher
- BioMed Central Ltd.
- Date published
- 2006
- DOI
- 10.1186/1471-2334-6-152
- ISSN
- 1471-2334
- Copyright note
- Copyright © 2006 Borel et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Organisation Unit
- University of the Sunshine Coast, Queensland; Centre for Bioinnovation
- Language
- English
- Record Identifier
- 99449331002621
- Output Type
- Journal article
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