Three forms of gonadotropin-releasing hormone in a perciform fish (Sparus aurata): complementary deoxyribonucleic acid characterization and brain localization

Y Gothilf; J A Munoz-Cueto; C A Sagrillo; M Selmanoff; T T Chen; O Kah; Abigail Elizur; Y Zohar

doi:10.1095/biolreprod55.3.636

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Three forms of gonadotropin-releasing hormone in a perciform fish (Sparus aurata): complementary deoxyribonucleic acid characterization and brain localization

Journal article

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Three forms of gonadotropin-releasing hormone in a perciform fish (Sparus aurata): complementary deoxyribonucleic acid characterization and brain localization

Y Gothilf, J A Munoz-Cueto, C A Sagrillo, M Selmanoff, T T Chen, O Kah, Abigail Elizur and Y Zohar

Biology of Reproduction, Vol.55(3), pp.636-645

1996

DOI: https://doi.org/10.1095/biolreprod55.3.636

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Abstract

Medical and Health Sciences

gonadotropin

Gilthead Seabream

Sparus aurata

Three forms of GnRH-salmon (sGnRH), seabream (sb-GnRH), and chicken (cGnRH-II)-have been described in the gilthead seabream (Sparus aurata) brain, and the cDNA encoding the sbGnRH precursor was recently isolated. In the present study, the cDNAs encoding the sGnRH and cGnRH- II were isolated and characterized, and the neurons producing the three GnRHs were localized in the seabream brain. Fragments of sGnRH and cGnRH-II cDNAs were amplified by polymerase chain reaction and used as probes to isolate the full-length cDNAs from a brain cDNA library. The cDNA encoding the cGnRH-II precursor is 573 nucleotides (nt) long, and the cDNA encoding the sGnRH precursor is 1971 nt in length with an unusually long 5' untranslated region. Specific single-strand DNA probes for in situ detection of mRNA were designed according to nonconserved regions among the three GnRH c-DNAs. Localization of GnRH mRNA-producing cells in the brain revealed five distinct populations of cells: sGnRH-producing cells in the ventromedial olfactory bulbs and the terminal nerve, sbGnRH-producing cells in the preoptic area and the ventral thalamus, and cGnRH-II-producing cells in the midbrain tegmentum. The discrete sites of expression of the three forms of GnRH indicate that only sbGnRH is directly involved in the control of gonadotropin secretion.

Details

Title: Three forms of gonadotropin-releasing hormone in a perciform fish (Sparus aurata): complementary deoxyribonucleic acid characterization and brain localization
Authors: Y Gothilf (Author) - Tel-Aviv University
J A Munoz-Cueto (Author) - Universidad de Cadiz
C A Sagrillo (Author)
M Selmanoff (Author)
T T Chen (Author)
O Kah (Author) - National Centre for Mariculture Israel
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Y Zohar (Author) - University of Maryland
Publication details: Biology of Reproduction, Vol.55(3), pp.636-645
Publisher: Society for the Study of Reproduction
Date published: 1996
DOI: 10.1095/biolreprod55.3.636
ISSN: 0006-3363
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449320402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Reproductive Biology

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