Three Forms of Gonadotropin-Releasing Hormone Characterized from Brains of One Species

J F F Powell; Y Zohar; Abigail Elizur; M Park; W H Fischer; A G Craig; J E Rivier; D A Lovejoy; N M Sherwood

doi:10.1073/pnas.91.25.12081

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Three Forms of Gonadotropin-Releasing Hormone Characterized from Brains of One Species

Journal article

Peer reviewed

Three Forms of Gonadotropin-Releasing Hormone Characterized from Brains of One Species

J F F Powell, Y Zohar, Abigail Elizur, M Park, W H Fischer, A G Craig, J E Rivier, D A Lovejoy and N M Sherwood

Proceedings of the National Academy of Sciences of the United States of America, Vol.91(25), pp.12081-12086

1995

DOI: https://doi.org/10.1073/pnas.91.25.12081

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Abstract

gonadotropin-releasing hormone

Most vertebrate species have more than one form of gonadotropin-releasing hormone (GnRH) in their brains, but it is not clear whether each form has a distinct function. We report that sea bream (Sparus aurata) brains have three forms of GnRH, one of which is described herein and is called sea bream GnRH (sbGnRH). The primary structures of two forms were determined by Edman degradation and mass spectral analysis. The amino acid sequence of sbGnRH is pGlu-His-Trp-Ser-Tyr-Gly-Len-Ser-Pro-Gly-NH2. The second peptide is identical to a form originally isolated from chicken brains (cGnRH-II): pGlu-His-Trp-Ser-His-Gly-Trp-Tyr-Pro-Gly-NH2. cGnRH-II is the most ancient form of GnRH identified to date in jawed fish and the most prevalent form throughout the vertebrates. The third form of GnRH has previously been identified as salmon GnRH by cDNA studies and is confirmed here by chromatographic and immunological studies. Phylogenetic distribution of GnRH peptides suggests sbGnRH arose in the perch-like fish as a gene duplication of the existing cGnRH-II or salmon GnRH genes. All three identified GnRH peptides were synthesized and shown to release gonadotropin in vivo in the sea bream. The dominant form of GnRH stored in the pituitary was sbGnRH. Not only was the content of sbGnRH 500-fold greater than that of salmon GnRH but also cGnRH-II was not detected in the pituitary. The latter evidence suggests that sbGnRH is the endogenous releaser of gonadotropin II.

Details

Title: Three Forms of Gonadotropin-Releasing Hormone Characterized from Brains of One Species
Authors: J F F Powell (Author) - University of Victoria
Y Zohar (Author) - University of Maryland
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
M Park (Author) - The Salk Institute, San Diego
W H Fischer (Author) - The Salk Institute, San Diego
A G Craig (Author) - The Salk Institute, San Diego
J E Rivier (Author) - The Salk Institute, San Diego
D A Lovejoy (Author) - University of Victoria
N M Sherwood (Author) - University of Victoria
Publication details: Proceedings of the National Academy of Sciences of the United States of America, Vol.91(25), pp.12081-12086
Publisher: National Academy of Sciences
Date published: 1995
DOI: 10.1073/pnas.91.25.12081
ISSN: 0027-8424
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449921402621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Endocrinology & Metabolism

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