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Thin layer coulometry of nitrite with ion-selective membranes
Journal article   Peer reviewed

Thin layer coulometry of nitrite with ion-selective membranes

M Afshar, G A Crespo, D Dorokhin, B Neel and Eric Bakker
Electroanalysis, Vol.27(3), pp.609-615
2015
DOI: https://doi.org/10.1002/elan.201400522
url
https://doi.org/10.1002/elan.201400522View
Published Version

Abstract

Analytical Chemistry Physical Chemistry (incl. Structural) Other Chemical Sciences thin layer coulometry ion-selective membranes nitrite detection universal detection systems urine analysis salophen
We report on a thin layer coulometric detection method based on an ion-selective membrane in view of a quantitative determination of nitrite in undiluted human urine and tap water. A cobalt(II) tert-butyl salophen compound, previously characterized by our group in ion-selective membrane electrodes, is used here as ionophore. The four orders of magnitude discrimination of chloride permits the detection of nitrite in samples that contain a large excess of chloride. The approach developed here allows one to determine nitrite within a concentration range of 20-100μM for a 120s current integration time. A modification of the reported electrochemical protocol to reduce the capacitive contribution diminishes the intercept of the calibration to -8.6μC. This is especially important in view of reaching calibration free systems. Initial in-house validation exhibits a limit of detection of 10μM in undiluted urine and in tap water. These values correspond to the regulation limits established in European Union and are adequate for the determination of nitrite for medical applications.

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Chemistry, Analytical
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