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Thin layer coulometry based on ion-exchanger membranes for heparin detection in undiluted human blood
Journal article   Peer reviewed

Thin layer coulometry based on ion-exchanger membranes for heparin detection in undiluted human blood

G A Crespo, M Ghahraman Afshar, D Dorokhin and Eric Bakker
Analytical Chemistry, Vol.86(3), pp.1357-1360
2014
DOI: https://doi.org/10.1021/ac403902f
url
https://doi.org/10.1021/ac403902fView
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Abstract

We explore here for the first time a potentially calibration-free methodology for the detection of protamine (and, by titration, heparin) in undiluted human blood in the therapeutic concentration range from 20 to 120 mg L-1. The use of a thin layer sample (5.8 μL) confined between a tubular protamine selective membrane (inner diameter, 600 μm) and a Ag/AgCl wire (diameter 400 μm) achieves an exhaustive depletion from the sample. Coulometry detection was chosen for the interrogation of the thin layer, employing a double pulse technique with 120 s for each pulse. Protamine calibration curves were recorded at physiological concentrations and in undiluted human blood. A linear relationship was obtained in both cases, but a diminished sensitivity was observed in contact with blood, which is explained with a partial passivation of the inner Ag/AgCl element. Heparin-protamine titrations were performed in undiluted human blood samples, mimicking the final application with patients undergoing critical care. The observed values correlate satisfactorily with those of an alternative technique, so-called flash-chronopotentiometry on planar membranes.

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Chemistry, Analytical
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