The search for mitochondrial tRNALeu(UUR) A3243G mutation among type 2 diabetes mellitus patients in the Nigerian population

Ameh S James; Imade Godwin; Ikenna Obi; Fabian Puepet; Bunza Aminu; Tsamiya Suleiman

doi:10.5897/AJB11.1556

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The search for mitochondrial tRNALeu(UUR) A3243G mutation among type 2 diabetes mellitus patients in the Nigerian population

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The search for mitochondrial tRNALeu(UUR) A3243G mutation among type 2 diabetes mellitus patients in the Nigerian population

Ameh S James, Imade Godwin, Ikenna Obi, Fabian Puepet, Bunza Aminu and Tsamiya Suleiman

African Journal of Biotechnology, Vol.10(62), pp.13383-13389

2011

DOI: https://doi.org/10.5897/AJB11.1556

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Abstract

Biological Sciences

Technology

maternal diabetes

mitochondrial gene

maternal inheritance and diabetic deafness

Nigeria

sub-Saharan Africa

The study aimed to compare the incidence of the pathogenic point mutation A3243G in the gene tRNALeu(UUR) indicating sub-type 2 diabetes mellitus conducted within the Nigerian population with that reported in other populations. 112patients diagnosed with type 2 diabetes (T2D) mellitus according to the World Health Organization criteria were selected based on family history and re-evaluated for associated disorders from the diabetic clinics in the Northern part of Nigeria. The mtDNA of these patients was extracted and the tRNALeu(UUR) gene screened for A3243G by PCR-RFLP method. Probands with maternal history were further investigated for other mutations using PCR-sequencing methods. None of the 112 patients were found to carry the A3243G mutation in the mitochondrial tRNALeu(UUR) gene in the homoplasmic or in the heteroplasmic form. However, C3254T was identified in two of our patients. This mutation was reported to be associated with gestational diabetes and linked with population from sub-Saharan Africa. The A3243G mutation in mitochondrial tRNALeu(UUR) is not a frequent cause of maternal diabetes in the Nigerian population contrary to other reported populations. However, further screening of an enlarged selected study group is necessary to fully determine the prevalence of this mutation in this population. This further search will help to fully appreciate the prevalence of maternal inheritance and diabetic deafness (MIDD) as extensively reported in other populations.

Details

Title: The search for mitochondrial tRNALeu(UUR) A3243G mutation among type 2 diabetes mellitus patients in the Nigerian population
Authors: Ameh S James (Author) - Family Health International, Nigeria
Imade Godwin (Author) - Jos University Teaching Hospital, Nigeria
Ikenna Obi (Author) - Apex Clinic, Nigeria
Fabian Puepet (Author) - Apex Clinic, Nigeria
Bunza Aminu (Author) - Sir Yaya Memorial Hospital, Nigeria
Tsamiya Suleiman (Author) - Yauri General Hospital, Nigeria
Publication details: African Journal of Biotechnology, Vol.10(62), pp.13383-13389
Publisher: Academic Journals
Date published: 2011
DOI: 10.5897/AJB11.1556
ISSN: 1684-5315
Copyright note: Copyright © 2011 Author(s) retain the copyright of this article. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License
Organisation Unit: School of Science and Engineering - Legacy; University of the Sunshine Coast, Queensland; GeneCology Research Centre - Legacy
Language: English
Record Identifier: 99449078602621
Output Type: Journal article

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