The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Guillaume Jannot; Sarah Bajan; Nellie J Giguère; Samir Bouasker; Isabelle H Banville; Sandra Piquet; Gyorgy Hutvagner; Martin J Simard

doi:10.1038/embor.2011.66

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The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Journal article

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The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans

Guillaume Jannot, Sarah Bajan, Nellie J Giguère, Samir Bouasker, Isabelle H Banville, Sandra Piquet, Gyorgy Hutvagner and Martin J Simard

EMBO reports, Vol.12(6), pp.581-586

2011

DOI: https://doi.org/10.1038/embor.2011.66

PMCID: PMC3128278

PMID: 21525958

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Abstract

Caenorhabditis elegans - metabolism

Caenorhabditis elegans - genetics

Humans

Gene Expression Regulation

Gene Silencing

Caenorhabditis elegans Proteins - metabolism

Receptors, Cell Surface - metabolism

MicroRNAs - metabolism

Neoplasm Proteins - metabolism

Animals

RNA-Induced Silencing Complex - metabolism

Receptors for Activated C Kinase

Polyribosomes - metabolism

MicroRNAs - genetics

HeLa Cells

GTP-Binding Proteins - metabolism

Protein Binding - physiology

Receptors, Cytoplasmic and Nuclear - metabolism

Despite the importance of microRNAs (miRNAs) in gene regulation, it is unclear how the miRNA-Argonaute complex--or miRNA-induced silencing complex (miRISC)--can regulate the translation of their targets in such diverse ways. We demonstrate here a direct interaction between the miRISC and the ribosome by showing that a constituent of the eukaryotic 40S subunit, receptor for activated C-kinase (RACK1), is important for miRNA-mediated gene regulation in animals. In vivo studies demonstrate that RACK1 interacts with components of the miRISC in nematodes and mammals. In both systems, the alteration of RACK1 expression alters miRNA function and impairs the association of the miRNA complex with the translating ribosomes. Our data indicate that RACK1 can contribute to the recruitment of miRISC to the site of translation, and support a post-initiation mode of miRNA-mediated gene repression.

Details

Title: The ribosomal protein RACK1 is required for microRNA function in both C. elegans and humans
Authors: Guillaume Jannot (Author) - Laval University Cancer Research Center
Sarah Bajan (Author) - University of Dundee
Nellie J Giguère (Author) - Laval University Cancer Research Center
Samir Bouasker (Author) - Laval University Cancer Research Center
Isabelle H Banville (Author) - Laval University Cancer Research Center
Sandra Piquet (Author) - Laval University Cancer Research Center
Gyorgy Hutvagner (Author) - University of Dundee
Martin J Simard (Author) - Laval University Cancer Research Center
Publication details: EMBO reports, Vol.12(6), pp.581-586
Publisher: Wiley-Blackwell Publishing Ltd.
Date published: 2011
DOI: 10.1038/embor.2011.66
ISSN: 1469-3178; 1469-221X
PMID: 21525958; PMC3128278
Copyright note: This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No DerivativeWorks 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
Organisation Unit: School of Health - Biomedicine; University of the Sunshine Coast, Queensland; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99472506002621
Output Type: Journal article

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Web Of Science research areas: Biochemistry & Molecular Biology; Cell Biology

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