The phylogeny of Ureaplasma urealyticum based on the mba gene fragment

Christine L Knox; Philip M Giffard; Peter Timms

doi:10.1099/00207713-48-4-1323

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The phylogeny of Ureaplasma urealyticum based on the mba gene fragment

Journal article

Open access

The phylogeny of Ureaplasma urealyticum based on the mba gene fragment

Christine L Knox, Philip M Giffard and Peter Timms

International Journal of Systematic Bacteriology, Vol.48(4), pp.1323-1331

1998

DOI: https://doi.org/10.1099/00207713-48-4-1323

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Abstract

Immunology

ureaplasma urealyticum

molecular subtyping

phylogeny

multiple banded antigen gene

urease gene

Sequencing of mba gene fragments of reference strains of Ureaplasma urealyticum serovars 1, 3, 6, 14, in addition to 33 clinical U. urealyticum isolates is reported. A phylogenetic tree deduced from an alignment of these sequences clearly demonstrates two major clusters (confidence limit 100%), which equate to the parvo and T960 biovars, and five types which we have designated mba 1, 3, 6, 8 and X. These relationships are supported by bootstrap analysis. Polymorphisms within the mba fragment of types mba 1, 3, and 6 were used to define nine subtypes (mba 1a, 1b, 3a, 3b, 3c, 3d, 3e, 6a, and 6b) thus facilitating high resolution typing of U. urealyticum. Inclusion of the reference strains for serovars 1, 3, 6, and 8 in the mba typing scheme showed that the results of this analysis are broadly consistent with currently accepted serotyping. In addition a ure gene fragment from nine of the clinical isolates was amplified and sequenced. Comparisons of the sequences clearly distinguished the two biovars of U. urealyticum; however this fragment was invariant within the parvo biovar. This study has shown that the sequence of the mba can reveal the fine details of the relationships between U. urealyticum isolates and also supports the significant evolutionary gap between the two biovars.

Details

Title: The phylogeny of Ureaplasma urealyticum based on the mba gene fragment
Authors: Christine L Knox (Author) - Queensland University of Technology
Philip M Giffard (Author) - Queensland University of Technology
Peter Timms (Author) - Queensland University of Technology
Publication details: International Journal of Systematic Bacteriology, Vol.48(4), pp.1323-1331
Publisher: Microbiology Society
Date published: 1998
DOI: 10.1099/00207713-48-4-1323
ISSN: 0020-7713
Copyright note: Copyright © 1998 The Author. The author's accepted version is reproduced here in accordance with the publisher's copyright policy. The definitive version is available at: http://www.microbiologyresearch.org/
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449151702621
Output Type: Journal article

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