The mRNA levels of GtH Iβ, GtH IIβ and GH in relation to testicular development and testosterone treatment in pituitary cells of male tilapia

P Melamed; G Gur; H Rosenfeld; Abigail Elizur; Z Yaron

doi:10.1023/A:1007737414565

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The mRNA levels of GtH Iβ, GtH IIβ and GH in relation to testicular development and testosterone treatment in pituitary cells of male tilapia

Journal article

Peer reviewed

The mRNA levels of GtH Iβ, GtH IIβ and GH in relation to testicular development and testosterone treatment in pituitary cells of male tilapia

P Melamed, G Gur, H Rosenfeld, Abigail Elizur and Z Yaron

Fish Physiology & Biochemistry, Vol.17, pp.93-98

1997

DOI: https://doi.org/10.1023/A:1007737414565

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Abstract

Ecology

Fisheries Sciences

Oreochromis niloticus

Oreochromis aureus

GtH Im

GtH II

mRNA

testosterone

testis

pituitary

The gonadotropin (GtH) II mRNA levels in pituitary glands of male tilapia hybrids were proportional to the gonadosomatic index (GSI), while GtH I was highest in fish with GSI of 0.56, but was lower in fish with more developed testes. In pituitary cells of immature fish, only GtH I mRNA responded to testosterone (T) exposure (12-72h), rising nearly three-fold compared to the control cells. The stimulatory effect of T on GtH I in these fish was also seen to be dose-related, but doses of 100 nM and above were ineffective. In pituitary cells of mature fish, both transcript levels rose on exposure to 0.01-0.1 nM T, but at higher concentrations, the GtH I fell to control levels, while those of GtH II continued to increase. Growth hormone (GH) mRNA levels were not affected by any of the T treatments. These results suggest that T may differentially control expression of the two GtH subunits; low levels in immature fish stimulating the I but not the II mRNA, while higher levels, in maturing fish, stimulate the II but not the I transcript.

Details

Title: The mRNA levels of GtH Iβ, GtH IIβ and GH in relation to testicular development and testosterone treatment in pituitary cells of male tilapia
Authors: P Melamed (Author) - Tel-Aviv University
G Gur (Author) - Tel-Aviv University
H Rosenfeld (Author) - National Centre for Mariculture Israel
Abigail Elizur (Author) - University of the Sunshine Coast - Faculty of Science, Health and Education
Z Yaron (Author) - Tel-Aviv University
Publication details: Fish Physiology & Biochemistry, Vol.17, pp.93-98
Publisher: Springer Netherlands
Date published: 1997
DOI: 10.1023/A:1007737414565
ISSN: 0920-1742
Organisation Unit: University of the Sunshine Coast, Queensland; Centre for Bioinnovation
Language: English
Record Identifier: 99449378002621
Output Type: Journal article

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