The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

Judit Remenyi; Sarah Bajan; Frances V Fuller-Pace; J Simon C Arthur; Gyorgy Hutvagner

doi:10.1038/srep22848

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The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

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The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132

Judit Remenyi, Sarah Bajan, Frances V Fuller-Pace, J Simon C Arthur and Gyorgy Hutvagner

Scientific Reports, Vol.6(1), pp.1-13

2016

DOI: https://doi.org/10.1038/srep22848

PMCID: PMC4780006

PMID: 26947125

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Abstract

Cell Line

Up-Regulation

RNA Processing, Post-Transcriptional

Humans

Gene Expression Regulation

Models, Molecular

MicroRNAs - metabolism

Animals

Mice

MicroRNAs - genetics

HeLa Cells

DEAD-box RNA Helicases - metabolism

Nucleic Acid Conformation

MicroRNAs - chemistry

miRNAs are small RNAs that are key regulators of gene expression in eukaryotic organisms. The processing of miRNAs is regulated by structural characteristics of the RNA and is also tightly controlled by auxiliary protein factors. Among them, RNA binding proteins play crucial roles to facilitate or inhibit miRNA maturation and can be controlled in a cell, tissue and species-specific manners or in response to environmental stimuli. In this study we dissect the molecular mechanism that promotes the overexpression of miR-132 in mice over its related, co-transcribed and co-regulated miRNA, miR-212. We have shown that the loop structure of miR-132 is a key determinant for its efficient processing in cells. We have also identified a range of RNA binding proteins that recognize the loop of miR-132 and influence both miR-132 and miR-212 processing. The DEAD box helicase p72/DDX17 was identified as a factor that facilitates the specific processing of miR-132.

Details

Title: The loop structure and the RNA helicase p72/DDX17 influence the processing efficiency of the mice miR-132
Authors: Judit Remenyi (Author) - University of Dundee
Sarah Bajan (Author) - University of Technology Sydney
Frances V Fuller-Pace (Corresponding Author) - University of Dundee
J Simon C Arthur (Corresponding Author) - University of Dundee
Gyorgy Hutvagner (Corresponding Author) - University of Technology Sydney
Publication details: Scientific Reports, Vol.6(1), pp.1-13
Publisher: Nature Publishing Group
Date published: 2016
DOI: 10.1038/srep22848
ISSN: 2045-2322
PMID: 26947125; PMC4780006
Copyright note: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Grant note: This work was supported by the Wellcome Trust CDF, EU FP6 SIROCCO project. G.H is an ARC Future Fellow. J.R. was funded by a grant from Breast Cancer Now (2010NOVPR16-previously known as Breast Cancer Campaign).
Organisation Unit: School of Health - Biomedicine; School of Health and Sport Sciences - Legacy; School of Health and Behavioural Sciences - Legacy
Language: English
Record Identifier: 99472506202621
Output Type: Journal article

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