The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock

Sankhya Bommana; Martina Jelocnik; Nicole Borel; Ian Marsh; Scott Carver; Adam Polkinghorne

doi:10.1099/jmm.0.000951

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The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock

Journal article

Peer reviewed

The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock

Sankhya Bommana, Martina Jelocnik, Nicole Borel, Ian Marsh, Scott Carver and Adam Polkinghorne

Journal of Medical Microbiology, Vol.68(4), pp.627-632

2019

DOI: https://doi.org/10.1099/jmm.0.000951

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Abstract

serology

peptide antigen based ELISA

chlamydia pecorum

ovine enzootic abortion

whole antigen based ELISA

chlamydia abortus

Chlamydia pecorum and Chlamydia abortus are related ruminant pathogens endemic to different global regions. Potential co-infections combined with the lack of species-specific serological assays challenge accurate diagnosis. Serological screening revealed low C. abortus seropositivity with the peptide-based ELISA (1/84; 1.2%) in Australian sheep yet moderate seropositivity in a Swiss flock with history of C. abortus-associated abortions (17/63; 26.9%). By whole cell antigen complement fixation tests (CFT) and ELISA, chlamydial seropositivity was significantly higher in all groups, suggesting cross-reactivity between these two chlamydial species and non-specificity of the tests. However, only C. pecorum DNA could be detected by qPCR in Chlamydia seropositive Australian animals screened, suggesting chlamydial seropositivity was due to cross-reactivity with endemic C. pecorum infections. These results suggest ascribing Chlamydia seropositivity to chlamydial species in livestock using whole-cell antigen CFT or ELISA should be treated with caution; and that peptide-based ELISA and qPCR provide greater chlamydial species-specificity.

Details

Title: The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock
Authors: Sankhya Bommana (Author) - University of the Sunshine Coast
Martina Jelocnik (Author) - University of the Sunshine Coast
Nicole Borel (Author) - University of Zurich, Switzerland
Ian Marsh (Author) - Elizabeth Macarthur Agricultural Institute
Scott Carver (Author) - University of Tasmania
Adam Polkinghorne (Author) - University of the Sunshine Coast
Publication details: Journal of Medical Microbiology, Vol.68(4), pp.627-632
Publisher: Microbiology Society
Date published: 2019
DOI: 10.1099/jmm.0.000951
ISSN: 0022-2615
Organisation Unit: University of the Sunshine Coast, Queensland; School of Science, Technology and Engineering; Centre for Bioinnovation
Language: English
Record Identifier: 99450773802621
Output Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Web Of Science research areas: Microbiology

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