The in vivo glycation of diabetic tendon collagen studied by neutron diffraction

Tim J Wess; L Wess; A Miller; R M Lindsay; J D Baird

doi:10.1006/jmbi.1993.1243

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$The in vivo glycation of diabetic tendon collagen studied by neutron diffraction$

Journal article

Peer reviewed

The in vivo glycation of diabetic tendon collagen studied by neutron diffraction

Tim J Wess, L Wess, A Miller, R M Lindsay and J D Baird

Journal of Molecular Biology, Vol.230(4), pp.1297-1303

1993

DOI: https://doi.org/10.1006/jmbi.1993.1243

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Abstract

collagen

cross-link

diabetic

glycation

neutrons

Glycation (non-enzymatic glycosylation) sites in the axial unit cell of diabetic tendon collagen were investigated by neutron Samples of diabetic and control tendon were reacted with sodium borodeuteride and sodium cyanoborodeuteride. This facilitated deuteration at aldimine, aldol or ketoimine groups in the molecule. These are natural collagen cross-links and sites where non-enzymatic glycation had occurred. The introduction of a deuteron at specific locations allowed the diabetic glycation collagen to be treated as multiple isomorphous derivatives for neutron fibre diffraction. Neutron diffraction was conducted at the Institut Laue Langevin, Grenoble. Standard crystallographic refinement techniques (modified for axial projections) were used to determine the structure of the control (non-diabetic) and diabetic samples. The results are shown as difference maps, these indicate that glycation takes place at different rates within the collagen axial unit cell. The position of glycation correlates well with the position of hydroxylysine residues. The reactions of periodate with enzymatically attached sugars, proteoglycan, natural cross-links and glycation products lead to complications in map interpretation. © Academic Press Limited.

Details

Title: The in vivo glycation of diabetic tendon collagen studied by neutron diffraction
Authors: Tim J Wess (Author) - University of Edinburgh, United Kingdom
L Wess (Author) - University of Edinburgh, United Kingdom
A Miller (Author) - University of Edinburgh, United Kingdom
R M Lindsay (Author) - University of Edinburgh, United Kingdom
J D Baird (Author) - University of Edinburgh, United Kingdom
Publication details: Journal of Molecular Biology, Vol.230(4), pp.1297-1303
Publisher: Academic Press
Date published: 1993
DOI: 10.1006/jmbi.1993.1243
ISSN: 0022-2836
Organisation Unit: Office of the Deputy Vice-Chancellor (Academic); University of the Sunshine Coast, Queensland
Language: English
Record Identifier: 99451331602621
Output Type: Journal article

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